pHsp70-Cas9

Plasmid for expressing S. pyogenes Cas9 in Drosophila cells from the hsp70 promoter. Use together with pU6-BbsI-chiRNA.

Sequence Author: O'Connor-Giles Lab / Addgene #45945

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NotI (6560) NotI (819) ATG SV40 NLS pHsp70-Cas9 8149 bp
NotI  (6560)
2 sites
G C G G C C G C C G C C G G C G
NotI  (819)
2 sites
G C G G C C G C C G C C G G C G
ATG
1309 .. 1311  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
1309 .. 1311  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
3xFLAG
1312 .. 1377  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
3xFLAG
1312 .. 1377  =  66 bp
22 amino acids  =  2.7 kDa
Product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site
SV40 NLS
1384 .. 1404  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
1384 .. 1404  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
Cas9
1429 .. 5529  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
1429 .. 5529  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
nucleoplasmin NLS
5530 .. 5577  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
nucleoplasmin NLS
5530 .. 5577  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from nucleoplasmin
hsp70 poly(A)
5657 .. 6529  =  873 bp
polyadenlyation signal from the Drosophila melanogaster hsp70Ab gene
hsp70 poly(A)
5657 .. 6529  =  873 bp
polyadenlyation signal from the Drosophila melanogaster hsp70Ab gene
NeoR/KanR
6742 .. 7536  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
6742 .. 7536  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
ori
182 .. 770  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
182 .. 770  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
hsp70 promoter
841 .. 1293  =  453 bp
Drosophila melanogaster hsp70Bb promoter
hsp70 promoter
841 .. 1293  =  453 bp
Drosophila melanogaster hsp70Bb promoter
ORF:  1309 .. 5580  =  4272 bp
ORF:  1423 amino acids  =  164.6 kDa
ORF:  6691 .. 7227  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  6054 .. 6662  =  609 bp
ORF:  202 amino acids  =  22.6 kDa
ORF:  6338 .. 6721  =  384 bp
ORF:  127 amino acids  =  14.0 kDa
ORF:  1234 .. 3945  =  2712 bp
ORF:  903 amino acids  =  95.3 kDa
ORF:  6742 .. 7536  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  516 .. 836  =  321 bp
ORF:  106 amino acids  =  11.8 kDa
ORF:  2775 .. 3068  =  294 bp
ORF:  97 amino acids  =  10.8 kDa
ORF:  3339 .. 3788  =  450 bp
ORF:  149 amino acids  =  17.3 kDa
ORF:  5982 .. 6497  =  516 bp
ORF:  171 amino acids  =  19.9 kDa
ORF:  6978 .. 7364  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
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Download pHsp70-Cas9.dna file

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