pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST

Level 1 hCas9 module plasmid for expressing human codon-optimized Cas9 from the CaMV 35S  promoter.

Sequence Author: Kamoun Lab / Addgene #49771

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SV40 NLS pICH47742::2x35S-5'UTR-hCas9(STOP)-NOST 9623 bp
Cas9
874 .. 4977  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
874 .. 4977  =  4104 bp
1368 amino acids  =  158.4 kDa
Product: Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
SV40 NLS
4990 .. 5010  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
4990 .. 5010  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
trfA
5506 .. 6654  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
trfA
5506 .. 6654  =  1149 bp
382 amino acids  =  43.8 kDa
Product: trans-acting replication protein that binds to and activates oriV
AmpR
7749 .. 8609  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   7749 .. 8540  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7749 .. 8609  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   8541 .. 8609  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
7749 .. 8609  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
oriV
8801 .. 9432  =  632 bp
incP origin of replication
oriV
8801 .. 9432  =  632 bp
incP origin of replication
ori
6990 .. 7578  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6990 .. 7578  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
CaMV 35S promoter
438 .. 783  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
CaMV 35S promoter
438 .. 783  =  346 bp
strong constitutive promoter from cauliflower mosaic virus
NOS terminator
5026 .. 5278  =  253 bp
nopaline synthase terminator and poly(A) signal
NOS terminator
5026 .. 5278  =  253 bp
nopaline synthase terminator and poly(A) signal
AmpR promoter
8610 .. 8714  =  105 bp
AmpR promoter
8610 .. 8714  =  105 bp
RB T-DNA repeat
5326 .. 5350  =  25 bp
right border repeat from nopaline C58 T-DNA
RB T-DNA repeat
5326 .. 5350  =  25 bp
right border repeat from nopaline C58 T-DNA
LB T-DNA repeat
9524 .. 9548  =  25 bp
left border repeat from nopaline C58 T-DNA
LB T-DNA repeat
9524 .. 9548  =  25 bp
left border repeat from nopaline C58 T-DNA
ORF:  367 .. 684  =  318 bp
ORF:  105 amino acids  =  11.7 kDa
ORF:  874 .. 5013  =  4140 bp
ORF:  1379 amino acids  =  159.7 kDa
ORF:  5518 .. 5913  =  396 bp
ORF:  131 amino acids  =  14.7 kDa
ORF:  7879 .. 8145  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  6125 .. 6712  =  588 bp
ORF:  195 amino acids  =  20.7 kDa
ORF:  9594 .. 357  =  387 bp
ORF:  128 amino acids  =  14.1 kDa
ORF:  9230 .. 41  =  435 bp
ORF:  144 amino acids  =  16.1 kDa
ORF:  2793 .. 3044  =  252 bp
ORF:  83 amino acids  =  9.9 kDa
ORF:  5793 .. 6065  =  273 bp
ORF:  90 amino acids  =  10.2 kDa
ORF:  6651 .. 6932  =  282 bp
ORF:  93 amino acids  =  10.4 kDa
ORF:  7749 .. 8609  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  5531 .. 5926  =  396 bp
ORF:  131 amino acids  =  14.1 kDa
ORF:  763 .. 1353  =  591 bp
ORF:  196 amino acids  =  21.9 kDa
ORF:  2278 .. 2796  =  519 bp
ORF:  172 amino acids  =  20.1 kDa
ORF:  2860 .. 3393  =  534 bp
ORF:  177 amino acids  =  19.9 kDa
ORF:  4387 .. 4920  =  534 bp
ORF:  177 amino acids  =  20.0 kDa
ORF:  5506 .. 6654  =  1149 bp
ORF:  382 amino acids  =  43.8 kDa
ORF:  6703 .. 6945  =  243 bp
ORF:  80 amino acids  =  9.0 kDa
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Download pICH47742_2x35S-5'UTR-hCas9(STOP)-NOST.dna file

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