pICSL01009_AtU6p

Level 0 vector encoding a consensus Arabidopsis U6 promoter for expressing a single guide RNA (sgRNA).

Sequence Author: Kamoun Lab / Addgene #46968

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No matches
BsaI (2224) BsaI (2148) pICSL01009::AtU6p 2323 bp
BsaI  (2224)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (2148)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
SmR
351 .. 1142  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
SmR
351 .. 1142  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase (Murphy, 1985)
confers resistance to spectinomycin and streptomycin
ori
1313 .. 1901  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
1313 .. 1901  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AtU6 promoter
2153 .. 2227  =  75 bp
consensus promoter from the three Arabidopsis U6 snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 2013)
AtU6 promoter
2153 .. 2227  =  75 bp
consensus promoter from the three Arabidopsis U6 snRNA genes (Waibel and Filipowicz, 1990; Nekrasov et al., 2013)
ORF:  132 .. 1142  =  1011 bp
ORF:  336 amino acids  =  37.5 kDa
ORF:  383 .. 640  =  258 bp
ORF:  85 amino acids  =  9.1 kDa
ORF:  650 .. 928  =  279 bp
ORF:  92 amino acids  =  9.4 kDa
ORF:  390 .. 773  =  384 bp
ORF:  127 amino acids  =  14.6 kDa
ORF:  864 .. 1103  =  240 bp
ORF:  79 amino acids  =  8.8 kDa
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Download pICSL01009_AtU6p.dna file

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