Resources
Plasmid Files

pL-CRISPR.SFFV.tRFP

Lentiviral CRISPR/Cas9 vector with a TagRFP marker driven by the SFFV promoter, for genome editing in mammalian cells.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pL-CRISPR.SFFV.tRFP Sequence and MappL-CRISPR.SFFV.tRFP.dna
Map and Sequence File   
Sequence Author:  Ebert Lab / Addgene #57826
Download Free Trial Get SnapGene Viewer

 BsmBI (1961) BsmBI (3846) polIII terminator ATG FLAG SV40 NLS pL-CRISPR.SFFV.tRFP 13,729 bp
BsmBI  (1961)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI  (3846)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
Cas9
4636 .. 8736  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
Cas9
4636 .. 8736  =  4101 bp
1367 amino acids  =  158.3 kDa
Product: Cas9 (Csn1) endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system
generates RNA-guided double strand breaks in DNA
spacer
1962 .. 3846  =  1885 bp
spacer
1962 .. 3846  =  1885 bp
AmpR
11,646 .. 12,506  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   11,646 .. 11,714  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
11,646 .. 12,506  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   11,715 .. 12,506  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
11,646 .. 12,506  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
TagRFP
8857 .. 9570  =  714 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent
protein from Entacmaea quadricolor (Merzlyak et
al., 2007)
mammalian codon-optimized
TagRFP
8857 .. 9570  =  714 bp
237 amino acids  =  26.7 kDa
Product: monomeric derivative of red fluorescent
protein from Entacmaea quadricolor (Merzlyak et
al., 2007)
mammalian codon-optimized
WPRE
9586 .. 10,174  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
WPRE
9586 .. 10,174  =  589 bp
woodchuck hepatitis virus posttranscriptional
regulatory element
ori
12,677 .. 13,265  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
12,677 .. 13,265  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
11,060 .. 11,515  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
11,060 .. 11,515  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
SFFV promoter
4145 .. 4546  =  402 bp
spleen focus-forming virus long terminal repeat
(LTR) promoter
SFFV promoter
4145 .. 4546  =  402 bp
spleen focus-forming virus long terminal repeat
(LTR) promoter
U6 promoter
1717 .. 1957  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
1717 .. 1957  =  241 bp
RNA polymerase III promoter for human U6 snRNA
RRE
1077 .. 1310  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1077 .. 1310  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
3' LTR (ΔU3)
10,246 .. 10,479  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
3' LTR (ΔU3)
10,246 .. 10,479  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
RSV promoter
5 .. 231  =  227 bp
Rous sarcoma virus enhancer/promoter
RSV promoter
5 .. 231  =  227 bp
Rous sarcoma virus enhancer/promoter
5' LTR (truncated)
232 .. 412  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
232 .. 412  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
SV40 ori
10,718 .. 10,853  =  136 bp
SV40 origin of replication
SV40 ori
10,718 .. 10,853  =  136 bp
SV40 origin of replication
HIV-1 Ψ
459 .. 584  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
459 .. 584  =  126 bp
packaging signal of human immunodeficiency virus
type 1
SV40 poly(A) signal
10,557 .. 10,678  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
10,557 .. 10,678  =  122 bp
SV40 polyadenylation signal
cPPT/CTS
3970 .. 4087  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
cPPT/CTS
3970 .. 4087  =  118 bp
central polypurine tract and central termination
sequence of HIV-1
AmpR promoter
11,541 .. 11,645  =  105 bp
AmpR promoter
11,541 .. 11,645  =  105 bp
gRNA scaffold
3847 .. 3922  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
3847 .. 3922  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
P2A
8800 .. 8856  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1
polyprotein
Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides.
P2A
8800 .. 8856  =  57 bp
19 amino acids  =  2.0 kDa
Product: 2A peptide from porcine teschovirus-1
polyprotein
Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides.
nucleoplasmin NLS
8737 .. 8784  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from
nucleoplasmin
nucleoplasmin NLS
8737 .. 8784  =  48 bp
16 amino acids  =  1.7 kDa
Product: bipartite nuclear localization signal from
nucleoplasmin
FLAG
4561 .. 4584  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
FLAG
4561 .. 4584  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an
enterokinase cleavage site
SV40 NLS
4591 .. 4611  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
SV40 NLS
4591 .. 4611  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T
antigen
polIII terminator
3923 .. 3928  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
3923 .. 3928  =  6 bp
RNA polymerase III transcription terminator
ATG
4558 .. 4560  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
4558 .. 4560  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter