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pLenti-Cas9

3rd-generation lentiviral vector for expressing Cas9 under control of the CMV promoter.

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pLenti-Cas9.dna
Map and Sequence File:    Download    Open   
Sequence Author:  OriGene
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BsmBI (9955) PasI (9948) ScaI (9766) Bsu36I (8436) BsrGI (7596) PmeI (7577) PaeR7I - PspXI - XhoI (7499) NotI (7493) BsiWI (7487) MluI (7484) BstEII (7427) XcmI (6868) PflFI - Tth111I (5902) SexAI * (5900) SgrDI (1) KasI (1017) NarI (1018) SfoI (1019) PluTI (1021) BssHII (1091) EcoNI (1550) BbvCI (1804) PstI (2417) PspOMI (2605) ApaI (2609) XbaI (2613) EcoRI (3279) BamHI (3292) AsiSI (3324) BstZ17I (3543) BlpI (4008) BfuAI - BspMI (4397) SphI (4589) PshAI (4904) pLenti-Cas9 10,530 bp
BsmBI  (9955)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PasI  (9948)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
ScaI  (9766)
1 site
A G T A C T T C A T G A
Bsu36I  (8436)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsrGI  (7596)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PmeI  (7577)
1 site
G T T T A A A C C A A A T T T G
PaeR7I  (7499)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (7499)
1 site
V C T C G A G B B G A G C T C V
XhoI  (7499)
1 site
C T C G A G G A G C T C
NotI  (7493)
1 site
G C G G C C G C C G C C G G C G
BsiWI  (7487)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
MluI  (7484)
1 site
A C G C G T T G C G C A
BstEII  (7427)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
XcmI  (6868)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PflFI  (5902)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5902)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
SexAI  (5900)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SgrDI  (1)
1 site
C G T C G A C G G C A G C T G C
KasI  (1017)
1 site
G G C G C C C C G C G G
NarI  (1018)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (1019)
1 site
G G C G C C C C G C G G
PluTI  (1021)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BssHII  (1091)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
EcoNI  (1550)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BbvCI  (1804)
1 site
C C T C A G C G G A G T C G
PstI  (2417)
1 site
C T G C A G G A C G T C
PspOMI  (2605)
1 site
G G G C C C C C C G G G
ApaI  (2609)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
XbaI  (2613)
1 site
T C T A G A A G A T C T
EcoRI  (3279)
1 site
G A A T T C C T T A A G
BamHI  (3292)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AsiSI  (3324)
1 site
G C G A T C G C C G C T A G C G
BstZ17I  (3543)
1 site
G T A T A C C A T A T G
BlpI  (4008)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BfuAI  (4397)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4397)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (4589)
1 site
G