Resources
Plasmid Files

pMB70

C. elegans plasmid for expressing a single guide RNA (sgRNA).

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pMB70 Sequence and MappMB70.dna
Map and Sequence File   
Sequence Author:  Boxem Lab / Addgene #47943
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 BsaI (188) BsaI (213) polIII terminator pMB70 3654 bp
BsaI  (188)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (213)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
3' CeU6
304 .. 1193  =  890 bp
region downstream of the C. elegans U6-1 snRNA
gene
3' CeU6
304 .. 1193  =  890 bp
region downstream of the C. elegans U6-1 snRNA
gene
NeoR/KanR
2265 .. 3059  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
2265 .. 3059  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
ori
1503 .. 2091  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1503 .. 2091  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CeU6 promoter
36 .. 192  =  157 bp
promoter for the C. elegans U6-1 snRNA gene
CeU6 promoter
36 .. 192  =  157 bp
promoter for the C. elegans U6-1 snRNA gene
AmpR promoter
3060 .. 3164  =  105 bp
AmpR promoter
3060 .. 3164  =  105 bp
gRNA scaffold
214 .. 289  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
214 .. 289  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
polIII terminator
290 .. 295  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
290 .. 295  =  6 bp
RNA polymerase III transcription terminator
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