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Plasmid Files

pME-Cas9

Middle entry Gateway® vector encoding zebrafish codon-optimized S. pyogenes Cas9 with nuclear localization signals.

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pME-Cas9.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Zon Lab / Addgene #63154
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BspLU11I - PciI - PscI (6901) BciVI - BfuI (6703) Alw44I - ApaLI - VneI (6587) BsmBI - Esp3I (5742) AsiSI - BpvUI - Ple19I - PvuI - RgaI - SfaAI - SgfI (5726) SspI (5651) Psp124BI - SacI - SstI (4971) Ecl136II - Eco53kI - EcoICRI (4969) BstXI (4963) AleI - OliI (4961) BseX3I - BstZI - CciNI - EagI - EclXI - Eco52I - NotI (4950) XbaI (4943) BglII (4905) AgeI - AsiGI - BshTI - CspAI - PinAI - SgrAI (4898) Acc113I - BmcAI - ScaI - ZrmI (4767) AarI (4760) AfeI - Aor51HI - Eco47III (4439) KflI - SanDI (4169) BalI - MlsI * - MluNI - MscI * - Msp20I (3948) BspQI - LguI - PciSI - SapI (111) AclI - Psp1406I (364) BstHPI - HpaI - KspAI (501) AhdI - AspEI - BmeRI - DriI - Eam1105I (549) AflII - BfrI - BspTI - Bst98I - BstAFI - MspCI - Vha464I (554) AbsI - PaeR7I - PspXI - Sfr274I - SlaI - XhoI (738) SalI (744) Bsp19I - NcoI (754) BglI (756) SbfI - SdaI - Sse8387I (1042) XcmI (1146) Acc16I - AviII - FspI - NsbI (1209) FauNDI - NdeI (1604) AxyI - Bse21I - Bsu36I - Eco81I (2061) DraI (2453) AdeI - DraIII (3191) CsiI - MabI - SexAI * (3363) pME-Cas9 6907 bp