pROSA26-Puro-DNR

Mammalian donor vector with a puromycin resistance marker for transgene integration at the ROSA26 locus in the mouse genome. Use together with pCas‑Guide-ROSA26.

Sequence Author: OriGene

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BsrGI (7805) MfeI (7149) BbvCI (6872) AleI (6646) FseI (6556) PmeI (6546) PspXI (6468) NotI (6462) MluI (6453) BmtI (6445) NheI (6441) AscI (6416) MreI - SgrAI (6412) AsiSI (6410) KpnI (6388) Acc65I (6384) BamHI (6378) AccI (6372) SalI (6371) EcoRI (6365) SacI (6312) Eco53kI (6310) NcoI (6104) SnaBI (6084) NdeI (5978) CMV enhancer PpuMI (5269) BfuAI - BspMI (5173) PflFI - Tth111I (5104) BstEII (5008) BstAPI (4579) BspDI - ClaI (4491) PflMI (4146) ApaI (3924) BspQI - SapI (319) PciI (435) SbfI (3787) PspOMI (3920) pROSA26-Puro-DNR 7810 bp
BsrGI  (7805)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
MfeI  (7149)
1 site
C A A T T G G T T A A C
BbvCI  (6872)
1 site
C C T C A G C G G A G T C G
AleI  (6646)
1 site
C A C N N N N G T G G T G N N N N C A C
FseI  (6556)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
PmeI  (6546)
1 site
G T T T A A A C C A A A T T T G
PspXI  (6468)
1 site
V C T C G A G B B G A G C T C V
NotI  (6462)
1 site
G C G G C C G C C G C C G G C G
MluI  (6453)
1 site
A C G C G T T G C G C A
BmtI  (6445)
1 site
G C T A G C C G A T C G
NheI  (6441)
1 site
G C T A G C C G A T C G
AscI  (6416)
1 site
G G C G C G C C C C G C G C G G
MreI  (6412)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (6412)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AsiSI  (6410)
1 site
G C G A T C G C C G C T A G C G
KpnI  (6388)
1 site
G G T A C C C C A T G G
Acc65I  (6384)
1 site
G G T A C C C C A T G G
BamHI  (6378)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AccI  (6372)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (6371)
1 site
G T C G A C C A G C T G
EcoRI  (6365)
1 site
G A A T T C C T T A A G
SacI  (6312)
1 site
G A G C T C C T C G A G
Eco53kI  (6310)
1 site
G A G C T C C T C G A G
NcoI  (6104)
1 site
C C A T G G G G T A C C
SnaBI  (6084)
1 site
T A C G T A A T G C A T
NdeI  (5978)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
PpuMI  (5269)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BfuAI  (5173)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (5173)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PflFI  (5104)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5104)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstEII  (5008)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BstAPI  (4579)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BspDI  (4491)
1 site
A T C G A T T A G C T A
ClaI  (4491)
1 site
A T C G A T T A G C T A
PflMI  (4146)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
ApaI  (3924)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BspQI  (319)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (319)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (435)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
SbfI  (3787)
1 site
C C T G C A G G G G A C G T C C
PspOMI  (3920)
1 site
G G G C C C C C C G G G
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   1255 .. 2046  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   2047 .. 2115  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
1255 .. 2115  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
HA-R
7161 .. 7810  =  650 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-R
7161 .. 7810  =  650 bp
right homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-L
3271 .. 3919  =  649 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
HA-L
3271 .. 3919  =  649 bp
left homology arm from intron 1 of the mouse Rosa26 gene (Soriano, 1999)
PuroR
4549 .. 5148  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
4549 .. 5148  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
496 .. 1084  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
496 .. 1084  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
PGK promoter
5188 .. 5687  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
5188 .. 5687  =  500 bp
mouse phosphoglycerate kinase 1 promoter
hGH poly(A) signal
6589 .. 7065  =  477 bp
human growth hormone polyadenylation signal
hGH poly(A) signal
6589 .. 7065  =  477 bp
human growth hormone polyadenylation signal
f1 ori
2502 .. 2957  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
2502 .. 2957  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
5805 .. 6108  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
5805 .. 6108  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
6109 .. 6312  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
6109 .. 6312  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
MCS
6364 .. 6558  =  195 bp
multiple cloning site
MCS
6364 .. 6558  =  195 bp
multiple cloning site
AmpR promoter
2116 .. 2220  =  105 bp
AmpR promoter
2116 .. 2220  =  105 bp
T7 promoter
6338 .. 6356  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
6338 .. 6356  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Myc
6471 .. 6500  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
Myc
6471 .. 6500  =  30 bp
10 amino acids  =  1.2 kDa
Product: Myc (human c-Myc proto-oncogene) epitope tag
FLAG
6519 .. 6542  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
FLAG
6519 .. 6542  =  24 bp
8 amino acids  =  1.0 kDa
Product: FLAG® epitope tag, followed by an enterokinase cleavage site
ORF:  2977 .. 3405  =  429 bp
ORF:  142 amino acids  =  15.8 kDa
ORF:  4498 .. 5175  =  678 bp
ORF:  225 amino acids  =  23.1 kDa
ORF:  1385 .. 1651  =  267 bp
ORF:  88 amino acids  =  9.3 kDa
ORF:  4460 .. 5407  =  948 bp
ORF:  315 amino acids  =  34.8 kDa
ORF:  1255 .. 2115  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  4549 .. 5148  =  600 bp
ORF:  199 amino acids  =  21.5 kDa
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Download pROSA26-Puro-DNR.dna file

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