Resources
Plasmid Files

pSQT1313

Multiplex guide RNA (gRNA) expression plasmid with cleavage sites for the Csy4 endoribonuclease.

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pSQT1313 Sequence and MappSQT1313.dna
Map and Sequence File   
Sequence Author:  Joung Lab / Addgene #53370
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 BsmBI (346) BsmBI (371) pSQT1313 2320 bp
BsmBI  (346)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI  (371)
2 sites
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
AmpR
1330 .. 2190  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   1330 .. 2121  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1330 .. 2190  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   2122 .. 2190  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
1330 .. 2190  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
571 .. 1159  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
571 .. 1159  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
U6 promoter
82 .. 322  =  241 bp
RNA polymerase III promoter for human U6 snRNA
U6 promoter
82 .. 322  =  241 bp
RNA polymerase III promoter for human U6 snRNA
AmpR promoter
2191 .. 2295  =  105 bp
AmpR promoter
2191 .. 2295  =  105 bp
gRNA scaffold
372 .. 447  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
372 .. 447  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
Csy4 site
336 .. 350  =  15 bp
cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)
Csy4 site
336 .. 350  =  15 bp
cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)
Csy4 site
453 .. 467  =  15 bp
cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)
Csy4 site
453 .. 467  =  15 bp
cleavage site for the Csy4 endoribonuclease
(Haurwitz et al., 2010)
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