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Plasmid Files

pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid for mammalian cell expression of Cas9 with the D10A nickase mutation and with an N-terminal nuclear localization signal followed by a FLAG® tag.

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pST1374-N-NLS-flag-linker-Cas9-D10A.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Huang Lab / Addgene #51130
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AhdI (8304) FspI (8081) SgrDI (7378) MfeI (7215) SpeI (7127) SnaBI (6790) SacI (6566) Eco53kI (6564) BmtI (6485) NheI (6481) NotI (6444) XbaI (6412) PflMI (6377) RsrII (6118) Bsu36I (5864) PaeR7I - XhoI (5176) BbvCI (5120) BamHI (4974) BstZ17I (258) MscI (943) TspMI - XmaI (1050) SmaI (1052) AvrII (1071) StuI (1076) SexAI * (1303) PmeI (2123) AgeI (2149) PspOMI (2206) ApaI (2210) KflI (2937) BstXI (3532) BmgBI (3807) EcoRV (4464) pST1374-N-NLS-flag-linker-Cas9-D10A 9317 bp