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pTDH3-dCas9-Mxi1

Yeast centromeric vector for expressing catalytically inactive dCas9 fused to the Mxi1 transcriptional repressor domain.

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pTDH3-dCas9-Mxi1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Qi & Weissman Labs / Addgene #46921
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EcoRI (106) BstHPI - HpaI - KspAI (10,356) BanIII - Bsa29I - BseCI - BshVI - BspDI - BspXI - Bsu15I - BsuTUI - ClaI (9794) AgeI - AsiGI - BshTI - CspAI - PinAI (9694) SmiI - SwaI (7646) AcvI - BbrPI - Eco72I - PmaCI - PmlI - PspCI (7622) AhdI - AspEI - BmeRI - DriI - Eam1105I (6689) PstI (5391) AhlI - BcuI - SpeI (117) BstSNI - Eco105I - SnaBI (166) Bsp19I - NcoI (814) ZraI (821) AatII (823) SgrDI (1215) BlpI - Bpu1102I - Bsp1720I - CelII (1528) BstAPI (1629) BsaBI * - Bse8I - BseJI * - MamI * (1666) AfeI - Aor51HI - Eco47III (2309) MluI (4682) HindIII (4992) PaeR7I - PspXI - Sfr274I - SlaI - XhoI (5145) BssNAI - Bst1107I - BstZ17I (5210) XbaI (5376) pTDH3-dCas9-Mxi1 11,211 bp
EcoRI  (106)
1 site
G A A T T C C T T A A G
BstHPI  (10,356)
1 site
G T T A A C C A A T T G
HpaI  (10,356)
1 site
G T T A A C C A A T T G
KspAI  (10,356)
1 site
G T T A A C C A A T T G
BanIII  (9794)
1 site
A T C G A T T A G C T A