Resources
Plasmid Files

pUC57-sgRNA expression vector

Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pUC57-sgRNA expression vector Sequence and MappUC57-sgRNA expression vector.dna
Map and Sequence File   
Sequence Author:  Huang Lab / Addgene #51132
Download Free Trial Get SnapGene Viewer

 M13 fwd BsaI (456) BsaI (481) polIII terminator pUC57-sgRNA expression vector 2792 bp
BsaI  (456)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaI  (481)
2 sites
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
KanR
1777 .. 2592  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418
(Geneticin®) in eukaryotes
KanR
1777 .. 2592  =  816 bp
271 amino acids  =  31.0 kDa
Product: aminoglycoside phosphotransferase
confers resistance to kanamycin in bacteria or G418
(Geneticin®) in eukaryotes
ori
1018 .. 1606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
1018 .. 1606  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
AmpR promoter
2593 .. 2697  =  105 bp
AmpR promoter
2593 .. 2697  =  105 bp
gRNA scaffold
482 .. 557  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
gRNA scaffold
482 .. 557  =  76 bp
guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system
T7 promoter
442 .. 460  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
442 .. 460  =  19 bp
promoter for bacteriophage T7 RNA polymerase
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 fwd
379 .. 395  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
615 .. 631  =  17 bp
common sequencing primer, one of multiple similar
variants
M13 rev
615 .. 631  =  17 bp
common sequencing primer, one of multiple similar
variants
polIII terminator
558 .. 563  =  6 bp
RNA polymerase III transcription terminator
polIII terminator
558 .. 563  =  6 bp
RNA polymerase III transcription terminator
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter