pdCas9-bacteria

Bacterial plasmid for tetracycline-inducible expression of catalytically dead S. pyogenes Cas9.

Sequence Author: Qi Lab / Addgene #44249

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pdCas9-bacteria 6705 bp
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   740 .. 766  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   767 .. 769  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   770 .. 3256  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   3257 .. 3259  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   3260 .. 4846  =  1587 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
740 .. 4846  =  4107 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
CmR
5920 .. 6579  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
5920 .. 6579  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
TetR
7 .. 630  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
TetR
7 .. 630  =  624 bp
207 amino acids  =  23.4 kDa
Product: tetracycline repressor TetR
TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline.
p15A ori
5146 .. 5691  =  546 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
p15A ori
5146 .. 5691  =  546 bp
Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.
cat promoter
6580 .. 6682  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
6580 .. 6682  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
lambda t0 terminator
5805 .. 5899  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
5805 .. 5899  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
4870 .. 4941  =  72 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
4870 .. 4941  =  72 bp
transcription terminator T1 from the E. coli rrnB gene
tetR/tetA promoters
649 .. 704  =  56 bp
overlapping promoters for bacterial tetR and tetA
tetR/tetA promoters
649 .. 704  =  56 bp
overlapping promoters for bacterial tetR and tetA
T7Te terminator
4957 .. 4984  =  28 bp
phage T7 early transcription terminator
T7Te terminator
4957 .. 4984  =  28 bp
phage T7 early transcription terminator
RBS
722 .. 733  =  12 bp
strong bacterial ribosome binding site (Elowitz and Leibler, 2000)
RBS
722 .. 733  =  12 bp
strong bacterial ribosome binding site (Elowitz and Leibler, 2000)
tet operator
685 .. 703  =  19 bp
bacterial operator O2 for the tetR and tetA genes
tet operator
685 .. 703  =  19 bp
bacterial operator O2 for the tetR and tetA genes
ORF:  740 .. 4846  =  4107 bp
ORF:  1368 amino acids  =  158.3 kDa
ORF:  5061 .. 5357  =  297 bp
ORF:  98 amino acids  =  10.9 kDa
ORF:  7 .. 633  =  627 bp
ORF:  208 amino acids  =  23.5 kDa
ORF:  5920 .. 6579  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  5724 .. 6002  =  279 bp
ORF:  92 amino acids  =  10.1 kDa
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