pdCas9-humanized

Retroviral vector encoding human codon-optimized catalytically dead dCas9 for CRISPR interference.

Sequence Author: Qi Lab / Addgene #44246

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SV40 NLS SV40 NLS SV40 NLS pdCas9-humanized 10,477 bp
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 1:  
   1423 .. 1449  =  27 bp
   9 amino acids  =  1.1 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 2:  
   1450 .. 1452  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 3:  
   1453 .. 3939  =  2487 bp
   829 amino acids  =  96.3 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 4:  
   3940 .. 3942  =  3 bp
   1 amino acid  =  89.1 Da
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
   Segment 5:  
   3943 .. 5526  =  1584 bp
   528 amino acids  =  60.9 kDa
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
dCas9
1423 .. 5526  =  4104 bp
1368 amino acids  =  158.3 kDa
5 segments
Product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain
SV40 NLS
5539 .. 5559  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5539 .. 5559  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5563 .. 5583  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5563 .. 5583  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5587 .. 5607  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
SV40 NLS
5587 .. 5607  =  21 bp
7 amino acids  =  883.1 Da
Product: nuclear localization signal of SV40 large T antigen
AmpR
8683 .. 9543  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   8683 .. 9474  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8683 .. 9543  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   9475 .. 9543  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8683 .. 9543  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
PuroR
6140 .. 6739  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
PuroR
6140 .. 6739  =  600 bp
199 amino acids  =  21.5 kDa
Product: puromycin N-acetyltransferase
confers resistance to puromycin
ori
7924 .. 8512  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
7924 .. 8512  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
5' LTR
10,476 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
5' LTR
10,476 .. 515  =  517 bp
5' long terminal repeat from murine embryonic stem cell virus
3' LTR
6839 .. 7353  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
3' LTR
6839 .. 7353  =  515 bp
3' long terminal repeat from murine embryonic stem cell virus
PGK promoter
5620 .. 6119  =  500 bp
mouse phosphoglycerate kinase 1 promoter
PGK promoter
5620 .. 6119  =  500 bp
mouse phosphoglycerate kinase 1 promoter
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
gag (truncated)
987 .. 1403  =  417 bp
truncated MMLV gag gene lacking the start codon
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
MESV Ψ
579 .. 920  =  342 bp
packaging signal of murine embryonic stem cell virus
AmpR promoter
9544 .. 9648  =  105 bp
AmpR promoter
9544 .. 9648  =  105 bp
ORF:  1423 .. 5610  =  4188 bp
ORF:  1395 amino acids  =  161.5 kDa
ORF:  5273 .. 5560  =  288 bp
ORF:  95 amino acids  =  10.3 kDa
ORF:  5900 .. 6739  =  840 bp
ORF:  279 amino acids  =  30.1 kDa
ORF:  8813 .. 9079  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  9929 .. 10,225  =  297 bp
ORF:  98 amino acids  =  11.7 kDa
ORF:  362 .. 637  =  276 bp
ORF:  91 amino acids  =  9.9 kDa
ORF:  674 .. 934  =  261 bp
ORF:  86 amino acids  =  9.5 kDa
ORF:  6065 .. 6790  =  726 bp
ORF:  241 amino acids  =  24.8 kDa
ORF:  3043 .. 3516  =  474 bp
ORF:  157 amino acids  =  17.4 kDa
ORF:  4828 .. 5214  =  387 bp
ORF:  128 amino acids  =  14.5 kDa
ORF:  8683 .. 9543  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
ORF:  3336 .. 3704  =  369 bp
ORF:  122 amino acids  =  14.1 kDa
ORF:  9993 .. 10,223  =  231 bp
ORF:  76 amino acids  =  7.5 kDa
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Download pdCas9-humanized.dna file

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