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Plasmid Files

CopGFP

Green fluorescent protein 2 from the copepod Pontellina plumata, also known as ppluGFP2.

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CopGFP Sequence and MapCopGFP.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 CopGFP End (669) ApaI - BaeGI - Bme1580I (569) EcoO109I - PspOMI (565) AcuI (564) BpmI (538) HincII (510) NruI (463) AclI (443) BsiHKAI (422) BtgI (413) XcmI (411) BsaWI (398) SfcI (306) BseRI (296) SphI (229) BmrI - KpnI (194) Acc65I (190) BciVI (163) HaeII - PluTI (131) SfoI (129) BsaHI - NarI (128) KasI (127) StyI (122) AccI (118) Bpu10I (86) Start (0) CopGFP 669 bp
End  (669)
0 sites
ApaI  (569)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BaeGI  (569)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (569)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
EcoO109I  (565)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PspOMI  (565)
1 site
G G G C C C C C C G G G
AcuI  (564)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (538)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
HincII  (510)
1 site
G T Y R A C C A R Y T G
NruI  (463)
1 site
T C G C G A A G C G C T
AclI  (443)
1 site
A A C G T T T T G C A A
BsiHKAI  (422)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BtgI  (413)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
XcmI  (411)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsaWI  (398)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
SfcI  (306)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BseRI  (296)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
SphI  (229)
1 site
G C A T G C C G T A C G
BmrI  (194)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
KpnI  (194)
1 site
G G T A C C C C A T G G
Acc65I  (190)
1 site
G G T A C C C C A T G G
BciVI  (163)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
HaeII  (131)
1 site
R G C G C Y Y C G C G R
PluTI  (131)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (129)
1 site
G G C G C C C C G C G G
BsaHI  (128)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (128)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (127)
1 site
G G C G C C C C G C G G
StyI  (122)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AccI  (118)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Bpu10I  (86)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Start  (0)
0 sites
CopGFP
1 .. 669  =  669 bp
222 amino acids  =  24.7 kDa
Product: green fluorescent protein 2 from Pontellina
plumata
, also known as ppluGFP2 (Shagin et al.,
2004)
CopGFP
1 .. 669  =  669 bp
222 amino acids  =  24.7 kDa
Product: green fluorescent protein 2 from Pontellina
plumata
, also known as ppluGFP2 (Shagin et al.,
2004)
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