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Plasmid Files

CyOFP1

Orange-red fluorescent protein that is excitable by cyan light and suitable as an acceptor for blue-emitting luciferase.

 
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 600 400 200 End (702) BsrGI - TatI (695) EciI - MmeI (692) PshAI (631) BsaAI - PflMI (585) BsaHI (575) HincII (528) AarI - BfuAI - BspMI (527) BmgBI (525) AcuI (517) BsgI (505) BssS α I (502) BglI (476) MspA1I (460) BmrI (433) SacI (356) Eco53kI (354) PfoI * (341) BpmI (325) BbsI (308) BstBI (299) Bsu36I (266) BsmI (172) BaeGI - Bme1580I (91) ApaLI (87) BseRI (31) BclI * (23) Start (0) CyOFP1 702 bp
End  (702)
0 sites
BsrGI  (695)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (695)
1 site
W G T A C W W C A T G W
EciI  (692)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
MmeI  (692)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PshAI  (631)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsaAI  (585)
1 site
Y A C G T R R T G C A Y
PflMI  (585)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsaHI  (575)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
HincII  (528)
1 site
G T Y R A C C A R Y T G
AarI  (527)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BfuAI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BmgBI  (525)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
AcuI  (517)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BssSαI  (502)
1 site
C A C G A G G T G C T C
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (460)
1 site
C M G C K G G K C G M C
BmrI  (433)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
SacI  (356)
1 site
G A G C T C C T C G A G
Eco53kI  (354)
1 site
G A G C T C C T C G A G
PfoI  (341)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (308)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BstBI  (299)
1 site
T T C G A A A A G C T T
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsmI  (172)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BaeGI  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (91)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (87)
1 site
G T G C A C C A C G T G
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
CyOFP1
1 .. 702  =  702 bp
234 amino acids  =  26.4 kDa
Product: orange-red fluorescent protein that is
excitable by cyan light and suitable as an acceptor
for blue-emitting luciferase (Chu et al., 2016)
CyOFP1
1 .. 702  =  702 bp
234 amino acids  =  26.4 kDa
Product: orange-red fluorescent protein that is
excitable by cyan light and suitable as an acceptor
for blue-emitting luciferase (Chu et al., 2016)
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