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Plasmid Files

E2-Red/Green

Noncytotoxic tetrameric dual-color red-and-green variant of DsRed fluorescent protein.

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E2-Red_Green Sequence and MapE2-Red_Green.dna
Map and Sequence File   
Sequence Author:  Glick Lab
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 600 400 200 End (678) AleI (628) BtgI (626) MscI (547) PflMI * (519) SexAI * (518) BstYI (479) PspFI (429) BseYI (425) BbsI (421) MmeI (416) PmlI (363) SbfI (341) BseRI (321) BmeT110I (263) AvaI - BsoBI (262) BmrI (183) AhdI (180) TaqII (136) AarI - BfuAI - BspMI (129) BssS α I (73) BsiHKAI (50) ApaLI (46) Start (0) E2-Red/Green 678 bp
End  (678)
0 sites
AleI  (628)
1 site
C A C N N N N G T G G T G N N N N C A C
BtgI  (626)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
MscI  (547)
1 site
T G G C C A A C C G G T
PflMI  (519)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
SexAI  (518)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BstYI  (479)
1 site
R G A T C Y Y C T A G R
PspFI  (429)
1 site
C C C A G C G G G T C G
BseYI  (425)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BbsI  (421)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
MmeI  (416)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PmlI  (363)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
SbfI  (341)
1 site
C C T G C A G G G G A C G T C C
BseRI  (321)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
BmeT110I  (263)
1 site
C Y C G R G G R G C Y C
AvaI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (262)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BmrI  (183)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
AhdI  (180)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
TaqII  (136)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
AarI  (129)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BfuAI  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (129)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BssSαI  (73)
1 site
C A C G A G G T G C T C
BsiHKAI  (50)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (46)
1 site
G T G C A C C A C G T G
Start  (0)
0 sites
E2-Red/Green
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: noncytotoxic tetrameric dual red-and-green
variant of DsRed fluorescent protein (Strack et al.,
2009)
mammalian codon-optimized
E2-Red/Green
1 .. 678  =  678 bp
225 amino acids  =  25.8 kDa
Product: noncytotoxic tetrameric dual red-and-green
variant of DsRed fluorescent protein (Strack et al.,
2009)
mammalian codon-optimized
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