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Plasmid Files

FAPα2

Fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment .

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FAPalpha2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  SpectraGenetics
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600 400 200 End (720) BspEI (373) BmgBI (282) BlpI (239) Start (0) FAP α2 720 bp
End  (720)
0 sites
BspEI  (373)
1 site
T C C G G A A G G C C T
BmgBI  (282)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BlpI  (239)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
Start  (0)
0 sites
FAPα2
1 .. 720  =  720 bp
240 amino acids  =  24.2 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
FAPα2
1 .. 720  =  720 bp
240 amino acids  =  24.2 kDa
Product: fluorogen-activating protein composed of a dimer of the L5-MG single-chain antibody fragment (Szent-Gyorgyi et al., 2008)
binds malachite green derivatives to produce red fluorescence
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.2 kDa  (no start codon)
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