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Plasmid Files

Gamillus

Acid-tolerant monomeric green fluorescent protein.

 
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Gamillus.dna
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600 400 200 Gamillus End (717) BsrGI (710) DraIII (657) SmaI (646) TspMI - XmaI (644) EcoO109I - PpuMI (630) StyI (590) EciI (584) AcuI (532) EcoNI (514) BglI (479) HaeII - PluTI (413) SfoI (411) BsaHI - NarI (410) KasI (409) Bsu36I (404) BssS α I (337) BsaWI (293) PfoI * (260) AflIII - PciI (221) BfuAI - BspMI (179) SgrAI (163) EcoP15I (156) ApoI (94) BanII - Bsp1286I (36) BseRI (31) Start (0) Gamillus 717 bp
End  (717)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
DraIII  (657)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SmaI  (646)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (644)
1 site
C C C G G G G G G C C C
XmaI  (644)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
EcoO109I  (630)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (630)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
StyI  (590)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EciI  (584)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
AcuI  (532)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoNI  (514)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BglI  (479)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
HaeII  (413)
1 site
R G C G C Y Y C G C G R
PluTI  (413)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (411)
1 site
G G C G C C C C G C G G
BsaHI  (410)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (410)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (409)
1 site
G G C G C C C C G C G G
Bsu36I  (404)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BssSαI  (337)
1 site
C A C G A G G T G C T C
BsaWI  (293)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI recognition sequence.
PfoI  (260)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
AflIII  (221)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (221)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BfuAI  (179)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (179)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SgrAI  (163)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EcoP15I  (156)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
ApoI  (94)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BanII  (36)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (36)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
Gamillus
1 .. 717  =  717 bp
239 amino acids  =  26.5 kDa
Product: acid-tolerant monomeric green fluorescent protein (Shinoda et al., 2018)
Gamillus
1 .. 717  =  717 bp
239 amino acids  =  26.5 kDa
Product: acid-tolerant monomeric green fluorescent protein (Shinoda et al., 2018)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  26.5 kDa
ORF:  3 .. 407  =  405 bp
ORF:  134 amino acids  =  15.6 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  23.7 kDa  (no start codon)
ORF:  282 .. 716  =  435 bp
ORF:  144 amino acids  =  16.3 kDa  (no start codon)
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