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Plasmid Files

IFP1.1

Infrared fluorescent protein derived from a bacterial phytochrome.

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IFP1.1 Sequence and MapIFP1.1.dna
Map and Sequence File   
Sequence Author:  Tsien Lab
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 800 600 400 200 End (966) BglII - BstYI (897) BsaAI (885) AhdI (859) BseRI (801) NspI (783) EciI (761) ApaI (642) PspOMI (638) BsaHI (542) ApoI (529) BsrGI - TatI (524) BsiEI - PvuI (513) HaeII (449) AfeI (447) XcmI (435) StuI * (391) BstBI (377) PfoI * (368) BanI - PspFI (326) BseYI - PflMI (322) BglI (256) EcoNI (187) BsmI (174) BsiHKAI (155) BsaWI - BspEI (142) BstXI (94) KflI - PpuMI (9) Start (0) IFP1.1 966 bp
End  (966)
0 sites
BglII  (897)
1 site
A G A T C T T C T A G A
BstYI  (897)
1 site
R G A T C Y Y C T A G R
BsaAI  (885)
1 site
Y A C G T R R T G C A Y
AhdI  (859)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BseRI  (801)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
NspI  (783)
1 site
R C A T G Y Y G T A C R
EciI  (761)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
ApaI  (642)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (638)
1 site
G G G C C C C C C G G G
BsaHI  (542)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
ApoI  (529)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BsrGI  (524)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (524)
1 site
W G T A C W W C A T G W
BsiEI  (513)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PvuI  (513)
1 site
C G A T C G G C T A G C
HaeII  (449)
1 site
R G C G C Y Y C G C G R
AfeI  (447)
1 site
A G C G C T T C G C G A
XcmI  (435)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
StuI  (391)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
BstBI  (377)
1 site
T T C G A A A A G C T T
PfoI  (368)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BanI  (326)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
PspFI  (326)
1 site
C C C A G C G G G T C G
BseYI  (322)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PflMI  (322)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BglI  (256)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
EcoNI  (187)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsmI  (174)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BsiHKAI  (155)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BsaWI  (142)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (142)
1 site
T C C G G A A G G C C T
BstXI  (94)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
KflI  (9)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (9)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Start  (0)
0 sites
IFP1.1
1 .. 966  =  966 bp
321 amino acids  =  34.8 kDa
Product: bacteriophytochrome-based infrared
fluorescent protein (Shu et al., 2009)
IFP1.1
1 .. 966  =  966 bp
321 amino acids  =  34.8 kDa
Product: bacteriophytochrome-based infrared
fluorescent protein (Shu et al., 2009)
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