IFP2.0

Monomeric infrared fluorescent protein derived from a bacterial phytochrome, engineered for improved brightness relative to IFP1.4.
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No matches
800 600 400 200 End (966) SbfI (944) BsaAI (885) AhdI (859) PvuII - MspA1I (846) Bsu36I (790) EciI (761) ApaI (642) PspOMI (638) BsgI (611) SphI (585) TatI BsrGI (524) BsiEI (513) PfoI - AcuI (491) BsrDI (434) NmeAIII (380) ApoI - EcoRI (373) BsaHI (284) EcoNI (187) CsiI - SexAI * (93) BsrBI (75) KflI - PpuMI (9) Start (0) IFP2.0 IFP2.0 966 bp
End  (966)
0 sites
SbfI  (944)
1 site
C C T G C A G G G G A C G T C C
BsaAI  (885)
1 site
Y A C G T R R T G C A Y
AhdI  (859)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PvuII  (846)
1 site
C A G C T G G T C G A C
MspA1I  (846)
1 site
C M G C K G G K C G M C
Bsu36I  (790)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (761)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
ApaI  (642)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
PspOMI  (638)
1 site
G G G C C C C C C G G G
BsgI  (611)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SphI  (585)
1 site
G C A T G C C G T A C G
TatI  (524)
1 site
W G T A C W W C A T G W
BsrGI  (524)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BsiEI  (513)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PfoI  (491)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
AcuI  (491)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrDI  (434)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
NmeAIII  (380)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ApoI  (373)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (373)
1 site
G A A T T C C T T A A G
BsaHI  (284)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoNI  (187)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
CsiI  (93)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (93)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
BsrBI  (75)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
KflI  (9)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (9)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Start  (0)
0 sites
IFP2.0
1 .. 966  =  966 bp
321 amino acids  =  34.9 kDa
Product: bacteriophytochrome-based monomeric infrared fluorescent protein (Yu et al., 2014)
IFP2.0
1 .. 966  =  966 bp
321 amino acids  =  34.9 kDa
Product: bacteriophytochrome-based monomeric infrared fluorescent protein (Yu et al., 2014)
ORF:  1 .. 966  =  966 bp
ORF:  321 amino acids  =  34.9 kDa
ORF:  2 .. 334  =  333 bp
ORF:  110 amino acids  =  12.4 kDa  (no start codon)
ORF:  502 .. 966  =  465 bp
ORF:  154 amino acids  =  16.2 kDa  (no start codon)
ORF:  111 .. 584  =  474 bp
ORF:  157 amino acids  =  16.4 kDa
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Download IFP2.0.dna file

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Individual Sequences & Maps

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