Kaede

Kaede green-to-red photoswitchable fluorescent protein.

Sequence Author: MBL International

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No matches
600 400 200 End (681) PflFI Tth111I (670) BsiHKAI (641) BtsI - BtsαI (582) AcuI - Eco57MI (504) HincII (461) EcoP15I (456) BbsI (412) BtgZI (291) BsmI (181) DrdI (130) BaeGI - Bme1580I * (65) BanI (62) BsrDI (57) Start (0) Kaede Kaede 681 bp
End  (681)
0 sites
PflFI  (670)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (670)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsiHKAI  (641)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BtsI  (582)
1 site
G C A G T G N N C G T C A C
BtsαI  (582)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AcuI  (504)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (504)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
HincII  (461)
1 site
G T Y R A C C A R Y T G
EcoP15I  (456)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgZI  (291)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
DrdI  (130)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G
* Blocked by EcoKI methylation.
Sticky ends from different Bme1580I sites may not be compatible.
BanI  (62)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsrDI  (57)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
Start  (0)
0 sites
Kaede
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: Kaede green-to-red photoconvertible fluorescent protein (Ando et al., 2002)
Kaede
1 .. 681  =  681 bp
226 amino acids  =  25.8 kDa
Product: Kaede green-to-red photoconvertible fluorescent protein (Ando et al., 2002)
ORF:  1 .. 681  =  681 bp
ORF:  226 amino acids  =  25.8 kDa
ORF:  1 .. 681  =  681 bp
ORF:  227 amino acids  =  26.3 kDa  (no start codon)
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Download Kaede.dna file

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Individual Sequences & Maps

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