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Plasmid Files

KillerRed

Red fluorescent protein KillerRed, a genetically-encoded photosensitizer.

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KillerRed Sequence and MapKillerRed.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 End (720) HaeII (698) BsaAI - PmlI (645) BstYI (575) NspI (453) PciI (449) AarI - BfuAI - BspMI (358) BanI (310) BsiEI (285) Bpu10I (274) EcoO109I (270) BmeT110I (266) AvaI - BsoBI (265) BanII (212) BsrFI (154) BsiHKAI (140) ApaLI (136) EcoP15I (135) Start (0) KillerRed 720 bp
End  (720)
0 sites
HaeII  (698)
1 site
R G C G C Y Y C G C G R
BsaAI  (645)
1 site
Y A C G T R R T G C A Y
PmlI  (645)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BstYI  (575)
1 site
R G A T C Y Y C T A G R
NspI  (453)
1 site
R C A T G Y Y G T A C R
PciI  (449)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AarI  (358)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI
recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its
electrophoretic mobility.
BfuAI  (358)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (358)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BanI  (310)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsiEI  (285)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
Bpu10I  (274)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
EcoO109I  (270)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BmeT110I  (266)
1 site
C Y C G R G G R G C Y C
AvaI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (265)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
BanII  (212)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsrFI  (154)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BsiHKAI  (140)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (136)
1 site
G T G C A C C A C G T G
EcoP15I  (135)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Start  (0)
0 sites
KillerRed
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
Product: red fluorescent protein KillerRed, a
genetically-encoded photosensitizer (Bulina et al.,
2006)
mammalian codon-optimized
KillerRed
1 .. 720  =  720 bp
239 amino acids  =  26.5 kDa
Product: red fluorescent protein KillerRed, a
genetically-encoded photosensitizer (Bulina et al.,
2006)
mammalian codon-optimized
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