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Plasmid Files

Kohinoor

Fast-switching photoswitchable fluorescent protein useful for fluorescence nanoscopy.

 
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 600 400 200 Kohinoor End (675) BsmI (645) NspI - SphI (637) MslI (632) DraIII (570) PsiI (530) MscI (500) EaeI (498) BtgZI (479) AcuI - Eco57MI (478) BspQI - SapI (454) ApoI (350) BbsI (293) Bsu36I (251) EciI (155) DrdI (127) XmnI (97) StyI (87) HaeII (50) Start (0) Kohinoor 675 bp
End  (675)
0 sites
BsmI  (645)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NspI  (637)
1 site
R C A T G Y Y G T A C R
SphI  (637)
1 site
G C A T G C C G T A C G
MslI  (632)
1 site
C A Y N N N N R T G G T R N N N N Y A C
DraIII  (570)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (530)
1 site
T T A T A A A A T A T T
MscI  (500)
1 site
T G G C C A A C C G G T
EaeI  (498)
1 site
Y G G C C R R C C G G Y
BtgZI  (479)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AcuI  (478)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (478)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BspQI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (454)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
ApoI  (350)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BbsI  (293)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (251)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EciI  (155)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
DrdI  (127)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
XmnI  (97)
1 site
G A A N N N N T T C C T T N N N N A A G
StyI  (87)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
HaeII  (50)
1 site
R G C G C Y Y C G C G R
Start  (0)
0 sites
Kohinoor
1 .. 675  =  675 bp
224 amino acids  =  25.3 kDa
Product: fast-switching photoswitchable fluorescent
protein useful for fluorescence nanoscopy (Tiwari et
al., 2015)
Kohinoor
1 .. 675  =  675 bp
224 amino acids  =  25.3 kDa
Product: fast-switching photoswitchable fluorescent
protein useful for fluorescence nanoscopy (Tiwari et
al., 2015)
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