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Plasmid Files

LSSmOrange

Monomeric orange fluorescent protein with a large Stokes shift, derived from mOrange.

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LSSmOrange Sequence and MapLSSmOrange.dna
Map and Sequence File   
Sequence Author:  Verkhusha Lab
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 600 400 200 End (711) BsrGI - TatI (701) NmeAIII (689) XcmI (687) BsrFI - SgrAI (682) AleI (643) HincII (600) AccI (599) SalI (598) BsgI (595) Bpu10I (502) BsrBI (459) BstXI (441) BbsI (436) BtgI - NcoI (434) PstI - SbfI (356) SfcI (352) SmlI (262) BpuEI (247) BsaAI (231) AhdI (195) BssS α I (88) BanII - Bsp1286I (76) FspI - FspAI (64) MscI (31) Start (0) LSSmOrange 711 bp
End  (711)
0 sites
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (701)
1 site
W G T A C W W C A T G W
NmeAIII  (689)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (682)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SgrAI  (682)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AleI  (643)
1 site
C A C N N N N G T G G T G N N N N C A C
HincII  (600)
1 site
G T Y R A C C A R Y T G
AccI  (599)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (598)
1 site
G T C G A C C A G C T G
BsgI  (595)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
Bpu10I  (502)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsrBI  (459)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
BstXI  (441)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BbsI  (436)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (434)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (434)
1 site
C C A T G G G G T A C C
PstI  (356)
1 site
C T G C A G G A C G T C
SbfI  (356)
1 site
C C T G C A G G G G A C G T C C
SfcI  (352)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
SmlI  (262)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (247)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (231)
1 site
Y A C G T R R T G C A Y
AhdI  (195)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BssSαI  (88)
1 site
C A C G A G G T G C T C
BanII  (76)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (76)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
FspI  (64)
1 site
T G C G C A A C G C G T
FspAI  (64)
1 site
R T G C G C A Y Y A C G C G T R
MscI  (31)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
LSSmOrange
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: monomeric orange fluorescent protein with
a large Stokes shift (Shcherbakova et al., 2012)
mammalian codon-optimized
LSSmOrange
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: monomeric orange fluorescent protein with
a large Stokes shift (Shcherbakova et al., 2012)
mammalian codon-optimized
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