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Plasmid Files

LanYFP

Tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3.

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LanYFP Sequence and MapLanYFP.dna
Map and Sequence File   
Sequence Author:  Allele Biotechnology
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 600 400 200 LanYFP End (660) StuI (640) TaqII (625) SmlI (595) BpuEI (580) EaeI (545) BstXI (540) HaeII (498) AfeI (496) MspA1I (403) EcoP15I (340) MmeI (323) BbsI (284) BsaWI - BspEI - BtgZI (244) BciVI (241) AhdI (210) BfuAI - BspMI (200) AlwNI (192) BamHI - BstYI (152) BaeGI - Bme1580I (135) StyI (125) BpmI (121) BseRI (118) PflMI (94) TsoI (86) BsrFI (79) PspFI (13) BseYI (9) Start (0) LanYFP 660 bp
End  (660)
0 sites
StuI  (640)
1 site
A G G C C T T C C G G A
TaqII  (625)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
SmlI  (595)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (580)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EaeI  (545)
1 site
Y G G C C R R C C G G Y
BstXI  (540)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
HaeII  (498)
1 site
R G C G C Y Y C G C G R
AfeI  (496)
1 site
A G C G C T T C G C G A
MspA1I  (403)
1 site
C M G C K G G K C G M C
EcoP15I  (340)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
MmeI  (323)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbsI  (284)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsaWI  (244)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (244)
1 site
T C C G G A A G G C C T
BtgZI  (244)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BciVI  (241)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
AhdI  (210)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BfuAI  (200)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (200)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AlwNI  (192)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BamHI  (152)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BstYI  (152)
1 site
R G A T C Y Y C T A G R
BaeGI  (135)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (135)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
StyI  (125)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BpmI  (121)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BseRI  (118)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
PflMI  (94)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
TsoI  (86)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (79)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (13)
1 site
C C C A G C G G G T C G
BseYI  (9)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
Start  (0)
0 sites
LanYFP
1 .. 660  =  660 bp
219 amino acids  =  24.7 kDa
Product: tetrameric yellow fluorescent protein from
Branchiostoma lanceolatum, also known as blFP-Y3
LanYFP
1 .. 660  =  660 bp
219 amino acids  =  24.7 kDa
Product: tetrameric yellow fluorescent protein from
Branchiostoma lanceolatum, also known as blFP-Y3
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