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Plasmid Files

PA-TagRFP

Bright photoactivatable variant of the monomeric red fluorescent protein TagRFP.

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PA-TagRFP Sequence and MapPA-TagRFP.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 End (702) PacI (699) HindIII (691) MscI (655) EaeI (653) DraIII (645) BsaI (616) BsaHI (572) SmlI (559) BpuEI (544) BstYI (535) AcuI (505) HincII (474) SalI (472) BglI (464) MspA1I (448) PspFI (420) BseYI (416) NmeAIII (393) PfoI * (329) BpmI (313) BbsI (296) Bsu36I (254) EcoP15I (225) PasI (219) TsoI (163) ApaLI (75) BsrGI - TatI (38) MslI (28) NspI (27) Start (0) PA-TagRFP 702 bp
End  (702)
0 sites
PacI  (699)
1 site
T T A A T T A A A A T T A A T T
HindIII  (691)
1 site
A A G C T T T T C G A A
MscI  (655)
1 site
T G G C C A A C C G G T
EaeI  (653)
1 site
Y G G C C R R C C G G Y
DraIII  (645)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsaI  (616)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsaHI  (572)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
SmlI  (559)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (544)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstYI  (535)
1 site
R G A T C Y Y C T A G R
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
HincII  (474)
1 site
G T Y R A C C A R Y T G
SalI  (472)
1 site
G T C G A C C A G C T G
BglI  (464)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
MspA1I  (448)
1 site
C M G C K G G K C G M C
PspFI  (420)
1 site
C C C A G C G G G T C G
BseYI  (416)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
NmeAIII  (393)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PfoI  (329)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BpmI  (313)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (296)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
EcoP15I  (225)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
PasI  (219)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TsoI  (163)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
ApaLI  (75)
1 site
G T G C A C C A C G T G
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (38)
1 site
W G T A C W W C A T G W
MslI  (28)
1 site
C A Y N N N N R T G G T R N N N N Y A C
NspI  (27)
1 site
R C A T G Y Y G T A C R
Start  (0)
0 sites
PA-TagRFP
1 .. 702  =  702 bp
233 amino acids  =  26.3 kDa
Product: photoactivatable variant of the monomeric
red fluorescent protein TagRFP (Subach et al., 2010)
mammalian codon-optimized
PA-TagRFP
1 .. 702  =  702 bp
233 amino acids  =  26.3 kDa
Product: photoactivatable variant of the monomeric
red fluorescent protein TagRFP (Subach et al., 2010)
mammalian codon-optimized
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