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Plasmid Files

TDsmURFP

 
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TDsmURFP.dna
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 800 600 400 200 End (870) BtgI - NcoI - StyI (467) MscI - MslI (466) BseRI (435) MspA1I (425) BsrBI (398) Start (0) TDsmURFP 870 bp
End  (870)
0 sites
BtgI  (467)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (467)
1 site
C C A T G G G G T A C C
StyI  (467)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (466)
1 site
T G G C C A A C C G G T
MslI  (466)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BseRI  (435)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
MspA1I  (425)
1 site
C M G C K G G K C G M C
BsrBI  (398)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
Start  (0)
0 sites
TDsmURFP
1 .. 870  =  870 bp
289 amino acids  =  32.0 kDa
Product: monomeric tandem dimer of a small
ultra-red fluorescent protein derived from a
cyanobacterial phycobiliprotein (Rodriguez et al.,
2016)
TDsmURFP
1 .. 870  =  870 bp
289 amino acids  =  32.0 kDa
Product: monomeric tandem dimer of a small
ultra-red fluorescent protein derived from a
cyanobacterial phycobiliprotein (Rodriguez et al.,
2016)
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