TagGFP2

Monomeric green fluorescent protein. Also known as mTagGFP.

Sequence Author: Evrogen

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No matches
600 400 200 End (717) AlwNI (677) EcoP15I (650) Bpu10I (601) BsaBI * (586) PasI (561) PvuII (531) AflIII (446) EciI (445) SacII (327) XmnI (244) BstAPI (206) BstEII (180) BsaHI (107) ApaLI (64) BmgBI (63) BseRI (28) NaeI (27) NgoMIV (25) BsrBI (5) Start (0) TagGFP2 TagGFP2 717 bp
End  (717)
0 sites
AlwNI  (677)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
EcoP15I  (650)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
Bpu10I  (601)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsaBI  (586)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
PasI  (561)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PvuII  (531)
1 site
C A G C T G G T C G A C
AflIII  (446)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
EciI  (445)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
SacII  (327)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
XmnI  (244)
1 site
G A A N N N N T T C C T T N N N N A A G
BstAPI  (206)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BstEII  (180)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsaHI  (107)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
ApaLI  (64)
1 site
G T G C A C C A C G T G
BmgBI  (63)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BseRI  (28)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
NaeI  (27)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (25)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BsrBI  (5)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
Start  (0)
0 sites
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
TagGFP2
1 .. 717  =  717 bp
238 amino acids  =  26.9 kDa
Product: monomeric green fluorescent protein, also known as mTagGFP
mammalian codon-optimized
ORF:  1 .. 717  =  717 bp
ORF:  238 amino acids  =  26.9 kDa
ORF:  3 .. 491  =  489 bp
ORF:  162 amino acids  =  18.5 kDa  (no start codon)
ORF:  1 .. 717  =  717 bp
ORF:  239 amino acids  =  23.6 kDa  (no start codon)
ORF:  273 .. 512  =  240 bp
ORF:  79 amino acids  =  9.4 kDa
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Download TagGFP2.dna file

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