Resources
Plasmid Files

TurboFP602

Red-shifted derivative of red fluorescent protein from Entacmaea quadricolor.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

TurboFP602 Sequence and MapTurboFP602.dna
Map and Sequence File   
Sequence Author:  Evrogen
Download Free Trial Get SnapGene Viewer

 600 400 200 End (708) PvuII (704) BmrI (703) ScaI (673) PshAI (640) BsaI (628) DraIII (594) BstYI (547) BfuAI - BspMI (527) Bts α I (522) AcuI (517) BsgI (505) BtgI - NcoI - StyI (480) BglI - SfiI (476) PspFI (432) BseYI (428) MmeI (419) NmeAIII (405) BpmI (325) BbsI (308) Bsu36I (266) PasI (231) TsoI (175) BsiHKAI (91) ApaLI (87) BsrGI (50) NspI (39) BclI * (23) Start (0) TurboFP602 708 bp
End  (708)
0 sites
PvuII  (704)
1 site
C A G C T G G T C G A C
BmrI  (703)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
ScaI  (673)
1 site
A G T A C T T C A T G A
PshAI  (640)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsaI  (628)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
DraIII  (594)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BstYI  (547)
1 site
R G A T C Y Y C T A G R
BfuAI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (527)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BtsαI  (522)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
AcuI  (517)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BtgI  (480)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (480)
1 site
C C A T G G G G T A C C
StyI  (480)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BglI  (476)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (476)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NmeAIII  (405)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (325)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BbsI  (308)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
PasI  (231)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
TsoI  (175)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsiHKAI  (91)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (87)
1 site
G T G C A C C A C G T G
BsrGI  (50)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
NspI  (39)
1 site
R C A T G Y Y G T A C R
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
TurboFP602
1 .. 708  =  708 bp
235 amino acids  =  26.3 kDa
Product: red-shifted derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
TurboFP602
1 .. 708  =  708 bp
235 amino acids  =  26.3 kDa
Product: red-shifted derivative of red fluorescent
protein from Entacmaea quadricolor
mammalian codon-optimized
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter