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Plasmid Files

TurboYFP

Enhanced derivative of PhiYFP.

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TurboYFP Sequence and MapTurboYFP.dna
Map and Sequence File   
Sequence Author:  Evrogen
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 600 400 200 TurboYFP End (732) BsaWI - BspEI (715) BssHII (672) BsaI (658) BlpI (619) ApaLI (577) BssS α I (499) BspQI - EarI - SapI (472) Bts α I (435) PspFI (406) BseYI - PflMI (402) NmeAIII (347) NspI - SphI (258) TsoI (254) XmnI (238) AleI (178) SgrAI (148) SacII (90) BsrDI (66) BstYI (32) Start (0) TurboYFP 732 bp
End  (732)
0 sites
BsaWI  (715)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (715)
1 site
T C C G G A A G G C C T
BssHII  (672)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BsaI  (658)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BlpI  (619)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
ApaLI  (577)
1 site
G T G C A C C A C G T G
BssSαI  (499)
1 site
C A C G A G G T G C T C
BspQI  (472)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
EarI  (472)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
SapI  (472)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
BtsαI  (435)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
PspFI  (406)
1 site
C C C A G C G G G T C G
BseYI  (402)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PflMI  (402)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NmeAIII  (347)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NspI  (258)
1 site
R C A T G Y Y G T A C R
SphI  (258)
1 site
G C A T G C C G T A C G
TsoI  (254)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (238)
1 site
G A A N N N N T T C C T T N N N N A A G
AleI  (178)
1 site
C A C N N N N G T G G T G N N N N C A C
SgrAI  (148)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
SacII  (90)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BsrDI  (66)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BstYI  (32)
1 site
R G A T C Y Y C T A G R
Start  (0)
0 sites
TurboYFP
1 .. 732  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
TurboYFP
1 .. 732  =  732 bp
243 amino acids  =  26.9 kDa
Product: enhanced derivative of PhiYFP
mammalian codon-optimized
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