YPet

Yellow fluorescent protein optimized for FRET with CPet.
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No matches
600 400 200 End (714) BsrGI (707) DrdI (652) BsaI (638) MflI * - BstYI (627) MfeI (560) BtsI - BtsαI (455) DraI (390) AcuI - Eco57MI (350) NdeI (230) MscI (171) EaeI (169) BstXI (167) BtgI - StyI - NcoI (166) BsaAI - BsiWI - SnaBI (113) BsaWI (88) Start (0) YPet YPet 714 bp
End  (714)
0 sites
BsrGI  (707)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
DrdI  (652)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsaI  (638)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
MflI  (627)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (627)
1 site
R G A T C Y Y C T A G R
MfeI  (560)
1 site
C A A T T G G T T A A C
BtsI  (455)
1 site
G C A G T G N N C G T C A C
BtsαI  (455)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
DraI  (390)
1 site
T T T A A A A A A T T T
AcuI  (350)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (350)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NdeI  (230)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
MscI  (171)
1 site
T G G C C A A C C G G T
EaeI  (169)
1 site
Y G G C C R R C C G G Y
BstXI  (167)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BtgI  (166)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
StyI  (166)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NcoI  (166)
1 site
C C A T G G G G T A C C
BsaAI  (113)
1 site
Y A C G T R R T G C A Y
BsiWI  (113)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (113)
1 site
T A C G T A A T G C A T
BsaWI  (88)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
Start  (0)
0 sites
YPet
1 .. 714  =  714 bp
238 amino acids  =  26.8 kDa
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
YPet
1 .. 714  =  714 bp
238 amino acids  =  26.8 kDa
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
ORF:  1 .. 714  =  714 bp
ORF:  238 amino acids  =  26.8 kDa
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Download YPet.dna file

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Individual Sequences & Maps

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