YPet (humanized)

Yellow fluorescent protein optimized for FRET with CyPet and for expression in mammalian cells.
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No matches
600 400 200 End (720) SacI - BanII (710) Eco53kI (708) NaeI (684) NgoMIV (682) PpuMI - EcoO109I (630) EcoNI (625) Bpu10I (604) PvuII MspA1I (534) MflI * BstYI (500) BsrGI (452) AflIII (449) PflMI (423) BsaWI (365) NmeAIII (356) SmaI (328) XmaI TspMI (326) BsaAI (279) KpnI (225) Acc65I (221) BtsI - BtsαI (214) PasI (192) BstEII (183) ApaLI (144) BsaHI (110) BmgBI (66) BstXI (48) SgrAI (28) EarI - SapI - BspQI (11) Start (0) YPet YPet (humanized) 720 bp
End  (720)
0 sites
SacI  (710)
1 site
G A G C T C C T C G A G
BanII  (710)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Eco53kI  (708)
1 site
G A G C T C C T C G A G
NaeI  (684)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (682)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
PpuMI  (630)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (630)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
EcoNI  (625)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (534)
1 site
C A G C T G G T C G A C
MspA1I  (534)
1 site
C M G C K G G K C G M C
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
BsrGI  (452)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
AflIII  (449)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PflMI  (423)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsaWI  (365)
1 site
W C C G G W W G G C C W

Cleavage may be enhanced when more than one copy of the BsaWI recognition sequence is present.
NmeAIII  (356)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SmaI  (328)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XmaI  (326)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
TspMI  (326)
1 site
C C C G G G G G G C C C
BsaAI  (279)
1 site
Y A C G T R R T G C A Y
KpnI  (225)
1 site
G G T A C C C C A T G G
Acc65I  (221)
1 site
G G T A C C C C A T G G
BtsI  (214)
1 site
G C A G T G N N C G T C A C
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
PasI  (192)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BstEII  (183)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
ApaLI  (144)
1 site
G T G C A C C A C G T G
BsaHI  (110)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BmgBI  (66)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstXI  (48)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SgrAI  (28)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
EarI  (11)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
YPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
YPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
YPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.7 kDa
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
YPet
1 .. 720  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: yellow fluorescent protein optimized for FRET with CyPet (Nguyen and Daugherty, 2005)
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  3 .. 716  =  714 bp
ORF:  237 amino acids  =  26.9 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.1 kDa  (no start codon)
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Download YPet (humanized).dna file

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