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Plasmid Files

hMGFP

Monster Green® fluorescent protein (Monster GFP), a modified version of Montastrea cavernosa GFP.

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hMGFP Sequence and MaphMGFP.dna
Map and Sequence File   
Sequence Author:  Promega
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 600 400 200 hMGFP End (684) NaeI (678) BglI (677) BsrFI - NgoMIV (676) BsiHKAI (644) DraIII - PflMI (579) BsgI (577) BstXI (552) BtgZI (488) AcuI - Bts α I - Eco57MI (487) BsaAI (450) BanII (431) BmrI (425) HincII (378) PflFI - Tth111I (373) BsrGI - TatI (353) BssS α I (261) BmeT110I (251) AvaI - BsoBI (250) StyI (215) BbsI (192) BstEII (190) AleI (175) ApoI (64) BclI * (8) Start (0) hMGFP 684 bp
End  (684)
0 sites
NaeI  (678)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
BglI  (677)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsrFI  (676)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
NgoMIV  (676)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BsiHKAI  (644)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
DraIII  (579)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PflMI  (579)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BsgI  (577)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (552)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BtgZI  (488)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AcuI  (487)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BtsαI  (487)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
Eco57MI  (487)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (450)
1 site
Y A C G T R R T G C A Y
BanII  (431)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BmrI  (425)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
HincII  (378)
1 site
G T Y R A C C A R Y T G
PflFI  (373)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (373)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsrGI  (353)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (353)
1 site
W G T A C W W C A T G W
BssSαI  (261)
1 site
C A C G A G G T G C T C
BmeT110I  (251)
1 site
C Y C G R G G R G C Y C
AvaI  (250)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (250)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
StyI  (215)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BbsI  (192)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BstEII  (190)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AleI  (175)
1 site
C A C N N N N G T G G T G N N N N C A C
ApoI  (64)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BclI  (8)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
hMGFP
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein
(Monster GFP), a modified version of the GFP from
Montastrea cavernosa
mammalian codon-optimized with few consensus
transcription factor binding sites
hMGFP
1 .. 684  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein
(Monster GFP), a modified version of the GFP from
Montastrea cavernosa
mammalian codon-optimized with few consensus
transcription factor binding sites
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