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Plasmid Files

hdKeima-Red

Humanized dimeric Keima-Red fluorescent protein.

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hdKeima-Red Sequence and MaphdKeima-Red.dna
Map and Sequence File   
Sequence Author:  MBL International
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 600 400 200 End (669) BstAPI (657) BsaBI * (640) Bts α I (634) EcoP15I (630) BtgZI (627) BtgI (620) AleI - MslI (619) BmgBI (591) AcuI (505) BsmI (461) BanII (425) BbsI (412) PasI (411) MspA1I (365) BaeGI - Bme1580I - BsiHKAI (307) ApaLI (303) HaeII - PluTI (299) SfoI (297) BsaHI - NarI (296) KasI (295) EcoNI (270) BsaAI (237) AhdI (171) StyI (137) NspI (45) AflIII - PciI (41) BsrGI - TatI (38) Start (0) hdKeima-Red 669 bp
End  (669)
0 sites
BstAPI  (657)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BsaBI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtsαI  (634)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
EcoP15I  (630)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BtgZI  (627)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BtgI  (620)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
AleI  (619)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (619)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmgBI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PasI  (411)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MspA1I  (365)
1 site
C M G C K G G K C G M C
BaeGI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsiHKAI  (307)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (303)
1 site
G T G C A C C A C G T G
HaeII  (299)
1 site
R G C G C Y Y C G C G R
PluTI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (297)
1 site
G G C G C C C C G C G G
BsaHI  (296)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (295)
1 site
G G C G C C C C G C G G
EcoNI  (270)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaAI  (237)
1 site
Y A C G T R R T G C A Y
AhdI  (171)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
NspI  (45)
1 site
R C A T G Y Y G T A C R
AflIII  (41)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (41)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (38)
1 site
W G T A C W W C A T G W
Start  (0)
0 sites
hdKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
Product: humanized dimeric Keima-Red fluorescent
protein
mammalian codon-optimized
hdKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
Product: humanized dimeric Keima-Red fluorescent
protein
mammalian codon-optimized
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