hmKeima-Red

Humanized monomeric Keima-Red fluorescent protein.

Sequence Author: MBL International

|Download SnapGene Viewer
No matches
600 400 200 End (669) BglI (662) BsaBI * (640) BtsI - BtsαI (634) BtgZI (627) AleI - MslI (619) BmgBI (591) BssSI - BssSαI (568) EagI (561) AcuI (505) BsmI (461) BbsI (412) PasI (411) MspA1I (365) BaeGI - Bme1580I (307) ApaLI (303) PluTI (299) SfoI (297) NarI - BsaHI (296) KasI (295) Bpu10I (172) StyI (137) BsrBI (47) TatI - BsrGI (38) Start (0) hmKeima-Red hmKeima-Red 669 bp
End  (669)
0 sites
BglI  (662)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaBI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtsI  (634)
1 site
G C A G T G N N C G T C A C
BtsαI  (634)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BtgZI  (627)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
AleI  (619)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (619)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BmgBI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BssSI  (568)
1 site
C A C G A G G T G C T C
BssSαI  (568)
1 site
C A C G A G G T G C T C
EagI  (561)
1 site
C G G C C G G C C G G C
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PasI  (411)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MspA1I  (365)
1 site
C M G C K G G K C G M C
BaeGI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (303)
1 site
G T G C A C C A C G T G
PluTI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (297)
1 site
G G C G C C C C G C G G
NarI  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (296)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (295)
1 site
G G C G C C C C G C G G
Bpu10I  (172)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
StyI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsrBI  (47)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
TatI  (38)
1 site
W G T A C W W C A T G W
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Start  (0)
0 sites
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 3:  
   7 .. 669  =  663 bp
   220 amino acids  =  24.8 kDa
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
hmKeima-Red
1 .. 669  =  669 bp
222 amino acids  =  25.1 kDa
3 segments
Product: humanized monomeric Keima-Red fluorescent protein
mammalian codon-optimized
ORF:  1 .. 669  =  669 bp
ORF:  222 amino acids  =  25.1 kDa
ORF:  3 .. 668  =  666 bp
ORF:  222 amino acids  =  25.7 kDa  (no start codon)
ORF:  1 .. 669  =  669 bp
ORF:  223 amino acids  =  21.6 kDa  (no start codon)
Click here to try SnapGene

Download hmKeima-Red.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.