hmKeima8.5

Human codon-optimized monomeric red fluorescent protein with a large Stokes shift.
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No matches
600 400 200 End (666) BsaBI * (640) BtgZI (627) BmgBI (591) BsiEI (579) BglI (535) AcuI (505) BsmI (461) NspI (441) BbsI (412) PasI (411) SacII (333) BtgI (330) BaeGI - Bme1580I (307) ApaLI (303) PluTI (299) SfoI (297) NarI - BsaHI (296) KasI (295) FspI (274) AvaI - BsoBI (253) Bpu10I (172) StyI (137) BsrBI (47) NmeAIII (41) TatI - BsrGI (38) Start (0) hmKeima8.5 hmKeima8.5 666 bp
End  (666)
0 sites
BsaBI  (640)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BtgZI  (627)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmgBI  (591)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsiEI  (579)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
BglI  (535)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
AcuI  (505)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BsmI  (461)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NspI  (441)
1 site
R C A T G Y Y G T A C R
BbsI  (412)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PasI  (411)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
SacII  (333)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BtgI  (330)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BaeGI  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (307)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
ApaLI  (303)
1 site
G T G C A C C A C G T G
PluTI  (299)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (297)
1 site
G G C G C C C C G C G G
NarI  (296)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (296)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (295)
1 site
G G C G C C C C G C G G
FspI  (274)
1 site
T G C G C A A C G C G T
AvaI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (253)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
Bpu10I  (172)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
StyI  (137)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BsrBI  (47)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
NmeAIII  (41)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
TatI  (38)
1 site
W G T A C W W C A T G W
BsrGI  (38)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Start  (0)
0 sites
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: human codon-optimized monomeric red fluorescent protein with a large Stokes shift (Guan et al. 2015)
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: human codon-optimized monomeric red fluorescent protein with a large Stokes shift (Guan et al. 2015)
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
3 segments
   Segment 3:  
   7 .. 666  =  660 bp
   220 amino acids  =  24.8 kDa
Product: human codon-optimized monomeric red fluorescent protein with a large Stokes shift (Guan et al. 2015)
hmKeima8.5
1 .. 666  =  666 bp
222 amino acids  =  25.1 kDa
3 segments
Product: human codon-optimized monomeric red fluorescent protein with a large Stokes shift (Guan et al. 2015)
ORF:  1 .. 666  =  666 bp
ORF:  222 amino acids  =  25.1 kDa
ORF:  3 .. 665  =  663 bp
ORF:  221 amino acids  =  25.8 kDa  (no start codon)
ORF:  1 .. 666  =  666 bp
ORF:  222 amino acids  =  21.4 kDa  (no start codon)
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Download hmKeima8.5.dna file

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Individual Sequences & Maps

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