hrGFP

Humanized Renilla reniformis green fluorescent protein.
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No matches
600 400 200 End (720) PasI (693) BsaI (649) BtgZI (648) XmnI (577) EarI - MslI - SapI - BspQI (550) FspI - FspAI (544) NspI (543) BsrGI (461) XcmI (453) BseRI (370) EcoNI (352) NaeI (279) NgoMIV (277) BanII Bsp1286I (199) Bpu10I (193) PluTI - HaeII (167) SfoI (165) NarI - BsaHI (164) KasI - BanI (163) BtgI - NcoI (95) PmlI (87) AflIII (86) BspHI * (44) AcuI (40) SbfI (38) AlwNI (18) Start (0) hrGFP hrGFP 720 bp
End  (720)
0 sites
PasI  (693)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BsaI  (649)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BtgZI  (648)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
XmnI  (577)
1 site
G A A N N N N T T C C T T N N N N A A G
EarI  (550)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
MslI  (550)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SapI  (550)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (550)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
FspI  (544)
1 site
T G C G C A A C G C G T
FspAI  (544)
1 site
R T G C G C A Y Y A C G C G T R
NspI  (543)
1 site
R C A T G Y Y G T A C R
BsrGI  (461)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
XcmI  (453)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BseRI  (370)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
EcoNI  (352)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
NaeI  (279)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
NgoMIV  (277)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
BanII  (199)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
Bsp1286I  (199)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Bpu10I  (193)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PluTI  (167)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
HaeII  (167)
1 site
R G C G C Y Y C G C G R
SfoI  (165)
1 site
G G C G C C C C G C G G
NarI  (164)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
BsaHI  (164)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
KasI  (163)
1 site
G G C G C C C C G C G G
BanI  (163)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BtgI  (95)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (95)
1 site
C C A T G G G G T A C C
PmlI  (87)
1 site
C A C G T G G T G C A C
AflIII  (86)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BspHI  (44)
1 site
T C A T G A A G T A C T
* Blocked by Dam methylation.
AcuI  (40)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Cleavage may be enhanced when more than one copy of the AcuI recognition sequence is present.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
SbfI  (38)
1 site
C C T G C A G G G G A C G T C C
AlwNI  (18)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
Start  (0)
0 sites
hrGFP
1 .. 720  =  720 bp
239 amino acids  =  27.3 kDa
Product: humanized Renilla green fluorescent protein
mammalian codon-optimized
hrGFP
1 .. 720  =  720 bp
239 amino acids  =  27.3 kDa
Product: humanized Renilla green fluorescent protein
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  27.3 kDa
ORF:  3 .. 719  =  717 bp
ORF:  239 amino acids  =  26.5 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.5 kDa  (no start codon)
ORF:  3 .. 263  =  261 bp
ORF:  87 amino acids  =  10.0 kDa
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Download hrGFP.dna file

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Individual Sequences & Maps

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