Resources
Plasmid Files

iRFP713

Phytochrome-based near-infrared fluorescent protein, originally termed iRFP.

To obtain this DNA and protein sequence with restriction sites, please download
 SnapGene or the free  SnapGene Viewer.

iRFP713 Sequence and MapiRFP713.dna
Map and Sequence File   
Sequence Author:  Verkhusha Lab
Download Free Trial Get SnapGene Viewer

 800 600 400 200 End (951) EarI (938) AlwNI * - PflMI * (918) NspI - SphI (792) ApoI - EcoRI (769) EcoRV (663) StuI (584) BsgI (571) SacII (464) BglI (463) BtgI (461) PstI - SbfI (428) SfcI (424) AatII (374) ZraI (372) PspFI (366) BseYI (362) SacI (353) Eco53kI (351) BclI * (330) BtgZI (307) SmaI (249) TspMI - XmaI (247) BbsI (192) BsrBI (185) PluTI (173) SfoI (171) NarI (170) KasI (169) BstXI (85) EcoP15I (63) BfuAI - BspMI (49) BamHI - BstYI (10) Start (0) iRFP713 951 bp
End  (951)
0 sites
EarI  (938)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
AlwNI  (918)
1 site
C A G N N N C T G G T C N N N G A C
* Blocked by Dcm methylation.
Sticky ends from different AlwNI sites may not be compatible.
PflMI  (918)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
NspI  (792)
1 site
R C A T G Y Y G T A C R
SphI  (792)
1 site
G C A T G C C G T A C G
ApoI  (769)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (769)
1 site
G A A T T C C T T A A G
EcoRV  (663)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
StuI  (584)
1 site
A G G C C T T C C G G A
BsgI  (571)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SacII  (464)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BglI  (463)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BtgI  (461)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
PstI  (428)
1 site
C T G C A G G A C G T C
SbfI  (428)
1 site
C C T G C A G G G G A C G T C C
SfcI  (424)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
AatII  (374)
1 site
G A C G T C C T G C A G
ZraI  (372)
1 site
G A C G T C C T G C A G
PspFI  (366)
1 site
C C C A G C G G G T C G
BseYI  (362)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
SacI  (353)
1 site
G A G C T C C T C G A G
Eco53kI  (351)
1 site
G A G C T C C T C G A G
BclI  (330)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BtgZI  (307)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
SmaI  (249)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (247)
1 site
C C C G G G G G G C C C
XmaI  (247)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
BbsI  (192)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsrBI  (185)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
PluTI  (173)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (171)
1 site
G G C G C C C C G C G G
NarI  (170)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
KasI  (169)
1 site
G G C G C C C C G C G G
BstXI  (85)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoP15I  (63)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BfuAI  (49)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (49)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BamHI  (10)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
BstYI  (10)
1 site
R G A T C Y Y C T A G R
Start  (0)
0 sites
iRFP713
1 .. 951  =  951 bp
316 amino acids  =  34.6 kDa
Product: phytochrome-based near-infrared
fluorescent protein, originally termed iRFP (Filonov
et al., 2011)
name was changed to iRFP713 (Shcherbakova and
Verkhusha, 2013)
iRFP713
1 .. 951  =  951 bp
316 amino acids  =  34.6 kDa
Product: phytochrome-based near-infrared
fluorescent protein, originally termed iRFP (Filonov
et al., 2011)
name was changed to iRFP713 (Shcherbakova and
Verkhusha, 2013)
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter