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Plasmid Files

mClavGR2

Monomeric photoconvertible green-to-red fluorescent protein derived from Clavularia cyan FP.

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mClavGR2 Sequence and MapmClavGR2.dna
Map and Sequence File   
Sequence Author:  Campbell Lab
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 600 400 200 End (714) BsrGI - TatI (704) BglI (689) BsiHKAI (665) Bpu10I (622) PflMI (600) BmgBI (495) BsaAI (468) EarI (421) BaeGI - Bme1580I (394) BtgZI (315) PvuII (290) SfcI (285) HaeII - PluTI (170) SfoI (168) BsaHI - NarI (167) BanI - KasI (166) FspI (64) BclI * (35) BspHI (26) BsaI (13) Start (0) mClavGR2 714 bp
End  (714)
0 sites
BsrGI  (704)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (704)
1 site
W G T A C W W C A T G W
BglI  (689)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsiHKAI  (665)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bpu10I  (622)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PflMI  (600)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BmgBI  (495)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BsaAI  (468)
1 site
Y A C G T R R T G C A Y
EarI  (421)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BaeGI  (394)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (394)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BtgZI  (315)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
PvuII  (290)
1 site
C A G C T G G T C G A C
SfcI  (285)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
HaeII  (170)
1 site
R G C G C Y Y C G C G R
PluTI  (170)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI
recognition sequence.
SfoI  (168)
1 site
G G C G C C C C G C G G
BsaHI  (167)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
NarI  (167)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI
recognition sequence.
BanI  (166)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
KasI  (166)
1 site
G G C G C C C C G C G G
FspI  (64)
1 site
T G C G C A A C G C G T
BclI  (35)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BspHI  (26)
1 site
T C A T G A A G T A C T
BsaI  (13)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
Start  (0)
0 sites
mClavGR2
1 .. 714  =  714 bp
237 amino acids  =  27.1 kDa
Product: monomeric photoconvertible green-to-red
fluorescent protein derived from Clavularia cyan FP
(Hoi et al., 2010)
mClavGR2
1 .. 714  =  714 bp
237 amino acids  =  27.1 kDa
Product: monomeric photoconvertible green-to-red
fluorescent protein derived from Clavularia cyan FP
(Hoi et al., 2010)
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