mEGFP

Enhanced GFP (EGFP) rendered monomeric by the A206K mutation.
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No matches
600 400 200 End (720) BsrGI (710) BlpI (622) BsiHKAI (610) BmrI (606) Bpu10I (604) MflI * - BstYI (500) PfoI * (281) BtsI - BtsαI (214) BssSI - BssSαI (181) BsrFI (151) BtgZI (122) BanI (36) BseRI (31) Start (0) mEGFP mEGFP 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsiHKAI  (610)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BmrI  (606)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
MflI  (500)
1 site
R G A T C Y Y C T A G R
* Blocked by Dam methylation.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsI  (214)
1 site
G C A G T G N N C G T C A C
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BssSI  (181)
1 site
C A C G A G G T G C T C
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Cleavage may be enhanced when more than one copy of the BsrFI recognition sequence is present.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
Start  (0)
0 sites
mEGFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: EGFP rendered monomeric by the A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
mEGFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.1 Da
Product: EGFP rendered monomeric by the A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
mEGFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.8 kDa
Product: EGFP rendered monomeric by the A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
mEGFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
Product: EGFP rendered monomeric by the A206K mutation (Zacharias et al., 2002)
mammalian codon-optimized
ORF:  1 .. 720  =  720 bp
ORF:  239 amino acids  =  27.0 kDa
ORF:  3 .. 719  =  717 bp
ORF:  239 amino acids  =  27.5 kDa  (no start codon)
ORF:  1 .. 720  =  720 bp
ORF:  240 amino acids  =  24.1 kDa  (no start codon)
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Download mEGFP.dna file

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Individual Sequences & Maps

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