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Plasmid Files

mEos4a

Fixation-resistant green-to-red photoswitchable fluorescent protein.

 
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 600 400 200 End (684) NspI - SphI (643) PflMI (576) Bpu10I (539) AcuI - Eco57MI (504) NdeI (476) BanII (425) KpnI (371) Acc65I (367) MslI (325) BstBI (287) HindIII (276) Bsu36I (254) BsmI (181) Bts α I (176) EcoRV (168) EciI (158) BstYI (123) BtgI - NcoI - StyI (119) BaeGI - Bme1580I (65) Start (0) mEos4a 684 bp
End  (684)
0 sites
NspI  (643)
1 site
R C A T G Y Y G T A C R
SphI  (643)
1 site
G C A T G C C G T A C G
PflMI  (576)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
Bpu10I  (539)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AcuI  (504)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Eco57MI  (504)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
NdeI  (476)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
BanII  (425)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
KpnI  (371)
1 site
G G T A C C C C A T G G
Acc65I  (367)
1 site
G G T A C C C C A T G G
MslI  (325)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BstBI  (287)
1 site
T T C G A A A A G C T T
HindIII  (276)
1 site
A A G C T T T T C G A A
Bsu36I  (254)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BsmI  (181)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BtsαI  (176)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
EcoRV  (168)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
EciI  (158)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BstYI  (123)
1 site
R G A T C Y Y C T A G R
BtgI  (119)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (119)
1 site
C C A T G G G G T A C C
StyI  (119)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BaeGI  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (65)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
Start  (0)
0 sites
mEos4a
1 .. 684  =  684 bp
227 amino acids  =  26.0 kDa
Product: fixation-resistant green-to-red
photoswitchable fluorescent protein (Paez-Segala et
al., 2015)
derived from mEos2
mEos4a
1 .. 684  =  684 bp
227 amino acids  =  26.0 kDa
Product: fixation-resistant green-to-red
photoswitchable fluorescent protein (Paez-Segala et
al., 2015)
derived from mEos2
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