mKO

Monomeric Kusabira-Orange fluorescent protein.

Sequence Author: MBL International

|Download SnapGene Viewer
No matches
600 400 200 End (657) BtgZI (574) BmrI (380) HpaI - HincII (378) ApoI (364) FspI - FspAI (323) BstBI (293) StuI - MmeI (266) EarI (221) PmlI - BsaAI (186) AhdI (177) StyI (143) MscI (142) MluI (129) MfeI (74) DrdI (52) Start (0) mKO mKO 657 bp
End  (657)
0 sites
BtgZI  (574)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BmrI  (380)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
HpaI  (378)
1 site
G T T A A C C A A T T G
HincII  (378)
1 site
G T Y R A C C A R Y T G
ApoI  (364)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
FspI  (323)
1 site
T G C G C A A C G C G T
FspAI  (323)
1 site
R T G C G C A Y Y A C G C G T R
BstBI  (293)
1 site
T T C G A A A A G C T T
StuI  (266)
1 site
A G G C C T T C C G G A
MmeI  (266)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EarI  (221)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
PmlI  (186)
1 site
C A C G T G G T G C A C
BsaAI  (186)
1 site
Y A C G T R R T G C A Y
AhdI  (177)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (143)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (142)
1 site
T G G C C A A C C G G T
MluI  (129)
1 site
A C G C G T T G C G C A
MfeI  (74)
1 site
C A A T T G G T T A A C
DrdI  (52)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
Start  (0)
0 sites
mKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange fluorescent protein (Karasawa et al., 2004)
mKO
1 .. 657  =  657 bp
218 amino acids  =  24.5 kDa
Product: monomeric Kusabira-Orange fluorescent protein (Karasawa et al., 2004)
ORF:  1 .. 657  =  657 bp
ORF:  218 amino acids  =  24.5 kDa
Click here to try SnapGene

Download mKO.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.