mMiCy1

Monomeric Midoriishi-Cyan fluorescent protein.

Sequence Author: MBL International

|Download SnapGene Viewer
No matches
600 400 200 End (699) HpaI (678) BbvCI Bpu10I (665) TaqII (628) StyI (618) AvaI - BsoBI (579) BsrDI (578) BstEII (520) PspFI (438) BseYI (434) MfeI (428) BpmI - Eco57MI (354) Esp3I - BsmBI (349) PvuII (332) BseRI (329) SfcI (323) NdeI (285) AhdI (189) Bsu36I (155) BclI * (143) AatII (133) ZraI (131) BsaHI (130) EcoNI (116) PvuI - BsiEI (89) BtsI - BtsαI (69) BtgZI (9) Start (0) mMiCy1 mMiCy1 699 bp
End  (699)
0 sites
HpaI  (678)
1 site
G T T A A C C A A T T G
BbvCI  (665)
1 site
C C T C A G C G G A G T C G
Bpu10I  (665)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
TaqII  (628)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
StyI  (618)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
AvaI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (579)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
BsrDI  (578)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
BstEII  (520)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspFI  (438)
1 site
C C C A G C G G G T C G
BseYI  (434)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
MfeI  (428)
1 site
C A A T T G G T T A A C
BpmI  (354)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Eco57MI  (354)
1 site
C T G R A G ( N ) 14 N N G A C Y T C ( N ) 14

Sticky ends from different Eco57MI sites may not be compatible.
After cleavage, Eco57MI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
Esp3I  (349)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
BsmBI  (349)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
PvuII  (332)
1 site
C A G C T G G T C G A C
BseRI  (329)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfcI  (323)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
NdeI  (285)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AhdI  (189)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
Bsu36I  (155)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
BclI  (143)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AatII  (133)
1 site
G A C G T C C T G C A G
ZraI  (131)
1 site
G A C G T C C T G C A G
BsaHI  (130)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
EcoNI  (116)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
PvuI  (89)
1 site
C G A T C G G C T A G C
BsiEI  (89)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
BtsI  (69)
1 site
G C A G T G N N C G T C A C
BtsαI  (69)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
BtgZI  (9)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
Start  (0)
0 sites
mMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: monomeric Midoriishi-Cyan fluorescent protein
mMiCy1
1 .. 699  =  699 bp
232 amino acids  =  26.0 kDa
Product: monomeric Midoriishi-Cyan fluorescent protein
ORF:  1 .. 699  =  699 bp
ORF:  232 amino acids  =  26.0 kDa
Click here to try SnapGene

Download mMiCy1.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.