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Plasmid Files

mNectarine

pH-sensitive monomeric red fluorescent protein derived from DsRed and mTangerine.

 
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 600 400 200 End (711) BsrGI - TatI (701) XcmI (687) BsrFI - SgrAI (682) AleI - MslI (643) HincII (600) AccI (599) SalI (598) BsgI (595) MspA1I - PvuII (579) DrdI (541) AlwNI (507) BsrBI (459) PspFI (444) BseYI (440) BtgI (434) Bts α I (430) PstI - SbfI (356) SfcI (352) MfeI (288) EcoO109I (282) AhdI (195) TaqII (151) BssS α I (88) BanII (76) BsiHKAI (65) ApaLI (61) MscI (31) Start (0) mNectarine 711 bp
End  (711)
0 sites
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (701)
1 site
W G T A C W W C A T G W
XcmI  (687)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (682)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SgrAI  (682)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AleI  (643)
1 site
C A C N N N N G T G G T G N N N N C A C
MslI  (643)
1 site
C A Y N N N N R T G G T R N N N N Y A C
HincII  (600)
1 site
G T Y R A C C A R Y T G
AccI  (599)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (598)
1 site
G T C G A C C A G C T G
BsgI  (595)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MspA1I  (579)
1 site
C M G C K G G K C G M C
PvuII  (579)
1 site
C A G C T G G T C G A C
DrdI  (541)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AlwNI  (507)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BsrBI  (459)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
PspFI  (444)
1 site
C C C A G C G G G T C G
BseYI  (440)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BtgI  (434)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
BtsαI  (430)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
PstI  (356)
1 site
C T G C A G G A C G T C
SbfI  (356)
1 site
C C T G C A G G G G A C G T C C
SfcI  (352)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
MfeI  (288)
1 site
C A A T T G G T T A A C
EcoO109I  (282)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AhdI  (195)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
TaqII  (151)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BssSαI  (88)
1 site
C A C G A G G T G C T C
BanII  (76)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (65)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (61)
1 site
G T G C A C C A C G T G
MscI  (31)
1 site
T G G C C A A C C G G T
Start  (0)
0 sites
mNectarine
1 .. 711  =  711 bp
236 amino acids  =  26.6 kDa
Product: pH-sensitive monomeric red fluorescent
protein derived from DsRed and mTangerine
(Johnson et al., 2009)
mammalian codon-optimized
mNectarine
1 .. 711  =  711 bp
236 amino acids  =  26.6 kDa
Product: pH-sensitive monomeric red fluorescent
protein derived from DsRed and mTangerine
(Johnson et al., 2009)
mammalian codon-optimized
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