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Plasmid Files

mNeonGreen

Bright monomeric yellow-green fluorescent protein derived from LanYFP.

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mNeonGreen Sequence and MapmNeonGreen.dna
Map and Sequence File   
Sequence Author:  Allele Biotechnology
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 600 400 200 mNeonGreen End (711) BsrGI - TatI (701) StuI (670) TaqII (655) SmlI (625) BpuEI (610) BsaAI (606) AflIII (605) BstXI (570) Bts α I (536) HaeII (528) AfeI (526) BsgI (465) BbsI (463) MspA1I (433) EcoP15I (370) HincII - HpaI (330) BspEI - BtgZI (274) BciVI (271) BtgI - NcoI (260) AhdI (240) AlwNI (222) BstEII (160) BpmI (151) PflMI (124) TsoI (116) BaeGI - Bme1580I - BsrFI (109) XcmI (78) PspFI (43) BseYI (39) Start (0) mNeonGreen 711 bp
End  (711)
0 sites
BsrGI  (701)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (701)
1 site
W G T A C W W C A T G W
StuI  (670)
1 site
A G G C C T T C C G G A
TaqII  (655)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
SmlI  (625)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
BpuEI  (610)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsaAI  (606)
1 site
Y A C G T R R T G C A Y
AflIII  (605)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BstXI  (570)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BtsαI  (536)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
HaeII  (528)
1 site
R G C G C Y Y C G C G R
AfeI  (526)
1 site
A G C G C T T C G C G A
BsgI  (465)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BbsI  (463)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
MspA1I  (433)
1 site
C M G C K G G K C G M C
EcoP15I  (370)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
HincII  (330)
1 site
G T Y R A C C A R Y T G
HpaI  (330)
1 site
G T T A A C C A A T T G
BspEI  (274)
1 site
T C C G G A A G G C C T
BtgZI  (274)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BciVI  (271)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BtgI  (260)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (260)
1 site
C C A T G G G G T A C C
AhdI  (240)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (222)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BstEII  (160)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BpmI  (151)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PflMI  (124)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
TsoI  (116)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
BaeGI  (109)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (109)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsrFI  (109)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
XcmI  (78)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
PspFI  (43)
1 site
C C C A G C G G G T C G
BseYI  (39)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
Start  (0)
0 sites
mNeonGreen
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: bright monomeric yellow-green fluorescent
protein derived from LanYFP (Shaner et al., 2013)
mNeonGreen
1 .. 711  =  711 bp
236 amino acids  =  26.7 kDa
Product: bright monomeric yellow-green fluorescent
protein derived from LanYFP (Shaner et al., 2013)
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