mRuby3

Monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability.
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No matches
600 400 200 End (711) TatI - BsrGI (704) BsaAI - SnaBI (642) BseRI (634) MslI (574) BtsI - BtsαI (522) MscI (515) BsgI (505) EcoP15I (488) BtgZI (474) BbsI (424) BpuEI (361) AccI (359) SacI BanII BsiHKAI Bsp1286I (356) Eco53kI (354) SmlI (340) Bsu36I (266) DraI (252) BsiEI (233) EagI (230) BmgBI (191) PpuMI - EcoO109I (157) BsiWI (113) HincII (69) TaqII (54) EarI - SapI - BspQI (11) Start (0) mRuby3 mRuby3 711 bp
End  (711)
0 sites
TatI  (704)
1 site
W G T A C W W C A T G W
BsrGI  (704)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BsaAI  (642)
1 site
Y A C G T R R T G C A Y
SnaBI  (642)
1 site
T A C G T A A T G C A T
BseRI  (634)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
MslI  (574)
1 site
C A Y N N N N R T G G T R N N N N Y A C
BtsI  (522)
1 site
G C A G T G N N C G T C A C
BtsαI  (522)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
MscI  (515)
1 site
T G G C C A A C C G G T
BsgI  (505)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EcoP15I  (488)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
BtgZI  (474)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpuEI  (361)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AccI  (359)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SacI  (356)
1 site
G A G C T C C T C G A G
BanII  (356)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (356)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
Bsp1286I  (356)
1 site
G D G C H C C H C G D G

Sticky ends from different Bsp1286I sites may not be compatible.
Eco53kI  (354)
1 site
G A G C T C C T C G A G
SmlI  (340)
1 site
C T Y R A G G A R Y T C

Cleavage may be enhanced when more than one copy of the SmlI recognition sequence is present.
Sticky ends from different SmlI sites may not be compatible.
Bsu36I  (266)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
DraI  (252)
1 site
T T T A A A A A A T T T
BsiEI  (233)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
EagI  (230)
1 site
C G G C C G G C C G G C
BmgBI  (191)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
PpuMI  (157)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
EcoO109I  (157)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsiWI  (113)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
HincII  (69)
1 site
G T Y R A C C A R Y T G
TaqII  (54)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
EarI  (11)
1 site
C T C T T C N G A G A A G N N N N

Cleavage may be enhanced when more than one copy of the EarI recognition sequence is present.
Sticky ends from different EarI sites may not be compatible.
SapI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BspQI  (11)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
Start  (0)
0 sites
mRuby3
1 .. 711  =  711 bp
237 amino acids  =  26.6 kDa
Product: monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability (Bajar et al., 2016)
mRuby3
1 .. 711  =  711 bp
237 amino acids  =  26.6 kDa
Product: monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability (Bajar et al., 2016)
ORF:  1 .. 711  =  711 bp
ORF:  237 amino acids  =  26.6 kDa
ORF:  1 .. 519  =  519 bp
ORF:  173 amino acids  =  19.4 kDa
ORF:  3 .. 239  =  237 bp
ORF:  79 amino acids  =  8.7 kDa
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Download mRuby3.dna file

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