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Plasmid Files

mScarlet

Bright monomeric red fluorescent protein evolved from a synthetic template.

 
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mScarlet.dna
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 600 400 200 End (696) BsrGI - TatI (689) XcmI (675) BsrFI - SgrAI (670) AleI (631) HincII (588) AccI (587) SalI (586) BsgI (583) MmeI (578) EciI (541) EagI - NotI (513) BsrBI (447) PspFI (432) BseYI (428) BbsI (424) SacII (378) MspA1I (377) EcoO109I * (212) AhdI (183) StyI (149) BstEII (144) TaqII (139) BssS α I (76) BanII (64) BsiHKAI (53) ApaLI (49) Bts α I (26) BclI * (23) Start (0) mScarlet 696 bp
End  (696)
0 sites
BsrGI  (689)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (689)
1 site
W G T A C W W C A T G W
XcmI  (675)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (670)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SgrAI  (670)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
HincII  (588)
1 site
G T Y R A C C A R Y T G
AccI  (587)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (586)
1 site
G T C G A C C A G C T G
BsgI  (583)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MmeI  (578)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
EciI  (541)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
EagI  (513)
1 site
C G G C C G G C C G G C
NotI  (513)
1 site
G C G G C C G C C G C C G G C G
BsrBI  (447)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
SacII  (378)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
MspA1I  (377)
1 site
C M G C K G G K C G M C
EcoO109I  (212)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
StyI  (149)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BstEII  (144)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
TaqII  (139)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BssSαI  (76)
1 site
C A C G A G G T G C T C
BanII  (64)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (53)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (49)
1 site
G T G C A C C A C G T G
BtsαI  (26)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BclI  (23)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
Start  (0)
0 sites
mScarlet
1 .. 696  =  696 bp
232 amino acids  =  26.4 kDa
Product: bright monomeric red fluorescent protein
evolved from a synthetic template (Bindels et al.,
2016)
mScarlet
1 .. 696  =  696 bp
232 amino acids  =  26.4 kDa
Product: bright monomeric red fluorescent protein
evolved from a synthetic template (Bindels et al.,
2016)
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