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Plasmid Files

mTangerine

Monomeric derivative of DsRed fluorescent protein.

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mTangerine Sequence and MapmTangerine.dna
Map and Sequence File   
Sequence Author:  Tsien Lab
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 600 400 200 End (696) BsrGI - TatI (689) XcmI (675) BsrFI - SgrAI (670) BssHII (648) AleI (631) BsgI (583) MspA1I - PvuII (567) MscI (550) BstXI (546) DrdI (529) BsrBI (447) PspFI (432) BseYI - PflMI (428) BbsI (424) BtgI - NcoI (422) MmeI (419) PstI - SbfI (344) SfcI (340) BsaAI (219) AhdI (183) BaeGI - Bme1580I (124) BssS α I (76) BanII (64) FspI - FspAI (52) Start (0) mTangerine 696 bp
End  (696)
0 sites
BsrGI  (689)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (689)
1 site
W G T A C W W C A T G W
XcmI  (675)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrFI  (670)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
SgrAI  (670)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
BssHII  (648)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
AleI  (631)
1 site
C A C N N N N G T G G T G N N N N C A C
BsgI  (583)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
MspA1I  (567)
1 site
C M G C K G G K C G M C
PvuII  (567)
1 site
C A G C T G G T C G A C
MscI  (550)
1 site
T G G C C A A C C G G T
BstXI  (546)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
DrdI  (529)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BsrBI  (447)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
PspFI  (432)
1 site
C C C A G C G G G T C G
BseYI  (428)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PflMI  (428)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BbsI  (424)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BtgI  (422)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (422)
1 site
C C A T G G G G T A C C
MmeI  (419)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PstI  (344)
1 site
C T G C A G G A C G T C
SbfI  (344)
1 site
C C T G C A G G G G A C G T C C
SfcI  (340)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BsaAI  (219)
1 site
Y A C G T R R T G C A Y
AhdI  (183)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BaeGI  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (124)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BssSαI  (76)
1 site
C A C G A G G T G C T C
BanII  (64)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
FspI  (52)
1 site
T G C G C A A C G C G T
FspAI  (52)
1 site
R T G C G C A Y Y A C G C G T R
Start  (0)
0 sites
mTangerine
1 .. 696  =  696 bp
232 amino acids  =  26.3 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Shaner et al., 2004)
mammalian codon-optimized
mTangerine
1 .. 696  =  696 bp
232 amino acids  =  26.3 kDa
Product: monomeric derivative of DsRed fluorescent
protein (Shaner et al., 2004)
mammalian codon-optimized
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