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Plasmid Files

miRFP703

Bright monomeric near-infrared fluorescent protein engineered from a bacterial phytochrome, with an emission maximum of 703 nm.

 
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miRFP703.dna
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800 600 400 200 End SmlI NruI * EcoO109I * AcuI MslI AlwNI PstI SfcI AflIII - PciI AatII ZraI HincII AccI SalI BsaAI PvuII BanII PflMI * BaeGI - Bme1580I - BsiHKAI ApaLI BamHI PluTI MreI - SgrAI SfoI NarI KasI BspDI * - ClaI * - PvuI EcoNI BsaWI - BspEI NsiI ScaI TatI SphI BsaBI * ApoI EarI BstBI BsmI BstAPI Start miRFP703 948 bp
End  (948)
0 sites
SmlI  (937)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
NruI  (919)
1 site
T C G C G A A G C G C T
* Blocked by Dam methylation.
EcoO109I  (834)
1 site
R G G N C C Y Y C C N G G R
* Blocked by Dcm methylation.
Sticky ends from different EcoO109I sites may not be compatible.
AcuI  (793)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
MslI  (784)
1 site
C A Y N N N N R T G G T R N N N N Y A C
AlwNI  (771)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PstI  (767)
1 site
C T G C A G G A C G T C
SfcI  (763)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
AflIII  (722)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (722)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AatII  (662)
1 site
G A C G T C C T G C A G
ZraI  (660)
1 site
G A C G T C C T G C A G
HincII  (657)
1 site
G T Y R A C C A R Y T G
AccI  (656)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SalI  (655)
1 site
G T C G A C C A G C T G
BsaAI  (630)
1 site
Y A C G T R R T G C A Y
PvuII  (624)
1 site
C A G C T G G T C G A C
BanII  (566)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PflMI  (534)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BaeGI  (484)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (484)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsiHKAI  (484)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (480)
1 site
G T G C A C C A C G T G
BamHI  (419)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PluTI  (406)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
MreI  (405)
1 site
C G C C G G C G G C G G C C G C
SgrAI  (405)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
SfoI  (404)
1 site
G G C G C C C C G C G G
NarI  (403)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (402)
1 site
G G C G C C C C G C G G
BspDI  (383)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (383)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
PvuI  (383)
1 site
C G A T C G G C T A G C
EcoNI  (338)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
BsaWI  (336)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI recognition sequence.
BspEI  (336)
1 site
T C C G G A A G G C C T
NsiI  (329)
1 site
A T G C A T T A C G T A
ScaI  (313)
1 site
A G T A C T T C A T G A
TatI  (311)
1 site
W G T A C W W C A T G W
SphI  (270)
1 site
G C A T G C C G T A C G
BsaBI  (232)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
ApoI  (166)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EarI  (62)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI recognition sequence.
Sticky ends from different EarI sites may not be compatible.
BstBI  (52)
1 site
T T C G A A A A G C T T
BsmI  (31)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BstAPI  (13)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
Start  (0)
0 sites
miRFP703
1 .. 948  =  948 bp
315 amino acids  =  34.5 kDa
Product: bright monomeric near-infrared fluorescent protein engineered from a bacterial phytochrome, with an emission maximum of 703 nm (Shcherbakova et al., 2016)
miRFP703
1 .. 948  =  948 bp
315 amino acids  =  34.5 kDa
Product: bright monomeric near-infrared fluorescent protein engineered from a bacterial phytochrome, with an emission maximum of 703 nm (Shcherbakova et al., 2016)
ORF:  1 .. 948  =  948 bp
ORF:  315 amino acids  =  34.5 kDa
ORF:  1 .. 330  =  330 bp
ORF:  110 amino acids  =  12.2 kDa
ORF:  436 .. 948  =  513 bp
ORF:  170 amino acids  =  19.4 kDa  (no start codon)
ORF:  132 .. 356  =  225 bp
ORF:  74 amino acids  =  7.9 kDa
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